Development Of A Compound Feed Additives With Anti-Fatigue Function For Canine

To study the influence of canine antifatigue functional food additive on the anti-mobility fatigue in policedogs, different canine antifatigue functional and nutritional food additive formulas were tested.It revealed that canine antifatiguc functional and nutritional food additive played a prominent role in improving policedog's cxcrcising and working ability and in promoting the restoring of policedog's fatigue.12 healthy German Shepherd dogs(6 male 6 female) were choosen for the study.In the process of the test,dog handlers ran with their dogs 1200M in the standard sporting runway at the averagc spccd of 4-6 km/h. The canine blood biochemistry indexes (red blood cell, whitcblood cell, hcmoglobin, total plasma protein, albumin, blood ghcosc, blood lactic acid, carbohydronate ion, urea nitrogen, blood creatinine,alkalinityphosphatasc, lactic dchydrogcnasc) were examined under the pcaccful condition and in 10rain, 1h, 2h and 4h after the movement, and the pulse, the body temperature, the breath rate and the 3 vain salivating quantity were determined simultaneously.According to the basic project defined in Functional Assessment Procedure and Test Methods of Health Food, which was issued by Chinese Sanitary Department, the rat loaded swimming was used to assess the anti-fatigue effect in this study. The L-Carnitine, the Ghtamine, thecreatine, Fructose-1,6-Diphosphate, ginseng, taurine as well as trace elements and vitamins were chooen as the raw material. The orthogonal test design method was employed in this study. The results demonstrated that the Ghtamine, Fructose-1,6-Diphosphate and ginseng could greatly influenced on the swimming endurence, and the L-Carnitine, thecreatine, taurine had a little influence on the swimming endurance. Therefore, the formula A1B2C2D2E2F2 was designed and applied to the test. The results revealed that the ability of mouse antifatigue, the anti-oxygen deficit, heat and cold resistant in the experiment group surpassed that of the comparison group.After dog's exercising, the mainly physiological indexes had changed. The change of the breath rate and the salivating quantity was prominent (P＜0.05), and the body temperature change was not remarkable. The blood biochemistry indexes also varied with the moving process. The quantity of red blood cell, white blood cell and hemoglobin elevated a little, the change of blood lactic acid and carbohydronate ion was remarkable, the concentration of blood lactic acid increased and that of carbohydronate ion decreased correspondingly. The blood glucose and lactic dehydrogenase also changed in the process of movement and the fatigue restoration. Therefore, the breath rate, the salivating quantity, the blood lactic, the carbohydronate ion, the blood glucose and the lactic dchydrogcnasc could be used as the indexes to evaluate the degree of dog's movement and fatigue restoration.Canine antifatigue functional food additive capsules were manufactured according to the formula A1B2C2D2E2F2. 10 healthy German shepherd dogs (4 male 6 female) were divided into two groups. Each group contained 2 male dogs and 3 female dogs. Every day only the experimental group was feed with 4 capsules, the test duration was 30 days. The body weight (limosis on the morning), the blood convention indexes (red blood cell, white blood cell, hemoglobin) and the biochemistry indexes (total protein, globulin) were tested beforc and after the cxpcrimcnt. After feeding with canine antifatigue functional food additive, the dog's bodywcight, the red blood cell and the hemoglobin had the tcndcncy to increase. The motility ability and the work performance had been enhanced remarkably. The duration of dog's playing and searching, the fatigue coming time and distance of 8km/h obliged motion remarkably surpasses the comparison group dog (P＜0.05).With the respect to the mobility fatigue restoration, after thc 8km/h obliged motion in jogging machine, the restoring time of dog's breath rate was shorter than that of the comparison group. 2h after the movement, the blood biochemistry indexes demonstrated that the restoring time of the blood glucose, the blood lactic acid, the carbohydronatc ion and thc lactic dchydrogcnase was better than that of the comparison group. But the variation of the urea nitrogcn and the alkalinityphosphatasc varied was not obvious.The application of this additive in 80 training dogs indicated that this additive had the remarkable effect in prolonging the high intensity training time under the high temperature environment and delaying the emerging of the mobility fatigue and physical stress reaction and so on becausc of the long distance transportation.