Preliminary Research On Tissue Culture Of Salvia Miltiorrhiza Bunge

Salvia miltiorrhiza Bunge is a kind of traditional and rare medicinal herbs. In these years, its new value of application is found unceasingly and its needs are increasing. So it should have widely applied prospect.This paper reports something about it .The callus was induced and proliferated by using young stem , leaf and leafstalk as explants .which came out in early spring . In this paper , its effects on induction and proliferation were discussed by using different kinds of explants and hormone and different physical and chemical factors.The growth circle and some physiological and biochemical factors were studied during the culture of callus, a set of rapid propagation techniques were founded, which was rapid, efficient and steady.All this provided scientific basis for tissue culture and secondary metabolism matters yield, and provided technical support for application of rapid propagation to practical production. The results are as follows:1. Effects on induction and proliferation of callusMedium MS+6-BA1. Omg/L+2,4-D0.5mg/L was the optimum medium for callus induction , and MS+6-BA1. 5mg/L+NAA0. 5mg/L was the optimum medium for callus proliferation. The optimum concentration of glucose was 20-40g/L and that of sucrose was 30g/L for callus proliferation.Medium MS did good to callus proliferation.2. Study on physiological and biochemical charactersThe growth circle of callus was 27 days and the growth curves looked like "S" under the condition cultured in light and darkThe growth circle could be divided in 3 parts: Delay(0-6d), Index (6-24d) and Steady (24-27d).During the growth circle , the tendency of soluble protein contents , SOD actives and POD actives were different during the 3 parts. They showed some physiology and biochemical characters during callus proliferation and cell actives. It was different between the growth curve of callus cultured in bright and that cultured in dark. 3. Study on rapid propagation techniqueMedium MS+6-BA1. Omg/L was the optimum medium for tufted bud induction and medium MS+6-BA1. 0mg/L+NAA0.05mg/L was the optimum medium for tufted bud proliferation with the 24 times breeding. Medium MS+6-BA0.5-2. 0mg/L was the optimum medium for adventitious bud induction and medium MS+6-BA1. Omg/L was the optimum medium for adventitious bud proliferation with the 15 times breeding.The optimum medium for rooting induction was 1/2 MS +IBA0. lmg/L...