Isolation, Identification Of Pathogeny And Develop On The Bi-combined Oil Inactivated Vaccine Against Pseudotuberculosis And Colibacillosis In Sheep And Goat

Colibacillosis in sheep and goat is an infection disease with diarrhea oracute septicemia caused by pathogenic E.coli. The lambs of days to sixmonths old are infected most easily. The mortality of septicemia typalColibacillosis in lambs is very high, which characters is high fever, laboredbreathing, nerves signs, pleura-pneumonia and acute death. The disease bringsabout huge loss to income of sheep and goat-raising. The twenty strains ofshort bacillus were isolated died lambs with typical clinical in various plants.The isolates all are gram negative and not hemolysis. Combinining the resultsof biochemical test and pathology signs together, it is indicated that thepathogenic bacterium is E.coli. Serological reaction test indentified that theserotypes of eighteen strains are O78. The isolates are resistance to penicillinand ampicillin, the different of sensibility to other drugs is very vast. Animalinfection test prove that the isolates can cause animal fall disease or death,and E.coli. same as nature case can be isolated from dead body of theseanimals.In recent years, Pseudotuberculosis in sheep and goat (CaseousLymphadenitis) is climbing up continually. It is the chronic zoonoticinfectious disease caused by Corynebacterium pseudotuberculosis. Thisinfection is often neglected by people because of its unnoticed clinicalsymptom and low mortality. It can cause some lymph nodes or organs caseousabscesses and bring about emaciation, poor doers, fetus die in uterus to sheepor goat. So, the disease is one of the infections to control and remove in theworld. The same ball-bacillus was isolated from cervix lymphatic abscess ofdiseased goats in some plants. It is gram positive without capsule and spore,resistant to Penicillin G and Rifampin etc.. It can cause experiment animalsfall in diseases or death, and can copy the same animal models as nature caseon goats. Combining results of biochemical test, serology test and clinicalsigns, it is concluded that the pathogenicity is Corynebacteriumpseudotuberculosis.In order to prevent and control colibacillosis and pseudotuberculosis, wetried to make the bi-combined oil inactivated vaccine from typical isolatedbacterium strains. After the isolated E.coli and Corynebacteriumpseudotuberculosis were cultured on suitable nutrition to proliferate andAbstract â…¢inactivated by Formaldehyde ,then add into adjuvant in proportion and makeinto the mutti-valent bi-combined oil inactivated vaccine of the two speciesbacteria. There are 10 billions E.coli CFU and Corynebacteriumpseudotuberculosis CFU in every milliliter vaccine. Laboratory tests indicatethat the vaccine is stable, effective and safe. Examining the lymphocytepercentages in WBC of goats in 28 days after inoculation and throughinfection test, the vaccine is proven that it have fine immunogenicity and therate of protection to all experiment animals is 100%. After the vaccine isinjected into about two hundred lambs of a plant in Yangling, one doubtfulcase have never been found in later 4 months. This bi-combined oilinactivated vaccine protected Colibacillosis of sheep and goat to outbreak andspread, also inhibited pseudotuberculosis as well. So the vaccine should beimproved and utilized extensively.