The Research Of Phylogenetic Relationship Of Eucommia Ulmoides From Different Geographical Origin In China

The sequence of the ITS region (including ITS-1, 5.8S rDNA and ITS-2) of nuclear ribosomal DNA of 15 species of Eucommia ulmoides Oliv. from different geographical region of China were determined. The results show that the size of the ITS region of Eucommia ulmoides Oliv. is from 587bp to 589bp (ITS-1 is from 218bp to 219bp, 5.8S rDNA is 164bp ,and ITS-2 is from 205bp to 206bp), and the aligned length of ITS region is 589 position which provides 5.41% variable sites and 1.65% phylogenetical informative sites when gaps are treated as missing,the variable sites are very stable, therefore ITS sequences would be suitable marker for the construction of Eucommia ulmoides Oliv. phylogeny. The length of 5.8S rDNA is very conservative in these species and there have no variable site. The content of G+C of ITS-1 region is from 63.93% to 65.74%,ITS-2 region is from 65.69% to 66.67%,and ITS region (including ITS-1,5.8S rDNA and ITS-2) is from 62.76% to 63.42%. On the basis of phylogenetic analysis of nucleotide sequences,the DNA sequence homologyes of Eucommia ulmoides Oliv. from different geographical region is above 96.9%,and the divergence is from 0.0 to 0.7%. Phylogram tree based on ITS sequence data indicates that the samples from same region is not always clustered, and the samples from different regions may be in same sectionï¼›on the other hand,it is not corresponding to the result that determined by the shape of leaf. Therefore, the rational phylogenetic relationship can be determined through associated these ITS data with other evidence.On the other hand we determined the 18S region of nuclear ribosomal DNA of Eucommia ulmoides Oliv.. The length of 18S region is 1609bp,and it have some distinction with the sequence of Eucommia ulmoides Oliv. from GenBank.