| Lespedeza bicolor was perennial defoliation forage shrub species oflegume trees. They were recognized for their advantages of fast-growing, high-yield of forage, richer nutritive value, tolerance to drought and barren, etc. Lespedeza bicolor which behave excellent in provenances experiment in the past were used as material , the rapid propagation techniques were studied ,and the physiological mechanisms of rooting were explored, try to provide the theoretical and realistic basement on Lespedeza bicolor physiological mechanisms meaning.(l)The green wood cutting was one rapid propagation techniques suit to Lespedeza bicolor.n green wood cutting ,it was the best that the cutting were cultivated in pearlstone : vermiculite: turfy soil(l:l:l); In order to increase the cutting survival rate , the cuttings were made of the upper of the current-year-old softwood branches in the end of June or in the begin of July, and that survival rate was 87%,it was higher than the ones made of the middle of and the base of softwood branches; Treated by NAAlOOmg/L for 2 hours, the survival rate was the highest; The cutting period was cultured device of half sun illumination and mistspray. That was the beat green wood cutting techniques for Lespedeza bicolor and the survival rate was over 87%(2)Hard wood cutting for Lespedeza bicolor was more difficult, and the survival rate was 0 treated by water.Using the suit technology was increase the survival rate. It was significant differences in survival rate cutting treated by different hormone and concentration, the best survival rate was 40% when 200mg/L NAA was used to treat cutting. The height of cutting showed significant differences after the fast-growth period. And the end of heitht of cutting treated by NAA100mg/L was 199cm,and was 20% higher than other treatments.(3)The root cutting for Lespedeza bicolor was most difficult, the cutting root could root the callus of the base end of the root cutting could form, but the sprout of the baseend of the root cutting was difficult to form.(4)The significant relation was between the nutritive materials and survival rate. There are positive correlation between the sugar content, C/N value and the survival rate, the index were above 0.99; We could select the cuttings according to the sugar content(5.44%)and C/N value (2.57)During the cutting root period, the change of sugar content was the same to C/N value.In the begin of cutting period ,the sugar content and C/N value were slowly increase ,and to the peak point after the 10 days; later, new rooting increasing, the sugar content and C/N value were decrease. And the change of sugar content was more remarkable than the check treatment, their peak point was earlier than the check treatment. It was showed that the translation of sugar in cuttings could be promoted by the hormone treatment, moreover the cutting survival rate increased.(5)The dynamic balances of endogenous hormone regulate adventitious rooting of the green wood cutting. Plant growth regulator treat could increase the contents of IAA, GA3, ABA and ZT. During the rooting, plant growth regulator treat could increase the contents of IAA, ZT and GA3, but decrease the levels of ABA; During the period of root primodium initiation and duration of root primodium ,the IAA/ZT and IAA/ABA were higher , After adventitious root forming, IAA/ZT and IAA/ABA were lower.Several cutting methods were studied, the technology of green wood cutting for Lespedeza bicolor were obtained, and the physiological mechanisms of rooting were researched further. These had great theoretical and realistic meaning. But the proportion of hormone and the physiological mechanisms of rooting, forming sprout at hard wood cutting and root cutting should be researched in-depth, in order to establish the mature clonal propagation technologic system and theory and promote the rapid propagation of Lespedeza bicolor...
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