Analysis Of Genetic Diversity By AFLP And Its Association With Body Measurements And Reproduction Performances In Seven Police Dog Breeds

In order to make sure that the police dog breeding base can supply good and enough trained police dog to police and army, AFLPs (amplified fragment length polymorphism) technique were applied to analyzing the genetic diversity of 221 individuals original from seven canine breeds including German shepherd dog, Rottweiler, Labrador retriever, English spring, Belgian malinois, Kunming dog and Doberman pinscher, and also the relationship between AFLP markers and measurements as well as reproduction performances of German shepherd dog and Rottweiler. The purposes of the present study were to set up an AFLP method for analysis of canine pooled DNA. Apply the AFLP to research of genetic diversity of seven policedog breeds and seek AFLP markers relative to the body measurements and reproduction performances of the dog on the basis of data from German shepherd dog and Rottweiler breed. Provide policedog breeding with gene marker assisted selection, gene localization and mapping.1 Analysis of genetic polymorphism in seven policedog breeds by AFLPsA total of 221 pitches were used in seven breeds, including German shepherd dog (n=4), Rottweiler (n=31), Labrador Retriever (n=30), English Spring (n=21), Belgian Malinois (n=45), Kunming dog (n=35) and Doberman pinscher (n=19). DNA samples were harvested from these seven breeds and were amplified by 25 selective primer combinations. The results showed that 21 of 25 selective primer combinations produced 5081 markers including 75 variable markers. Namely, each combination had 34.57 markers. Clustering analysis was performed on the basis of the markers of seven breeds by UPGMA, indicating that AFLP could be used to effectively analyse the genetic diffence among the seven breeds.2 The relationship between AFLP markers and body measurements as well as reproduction in German shepherd dogBlood DNA samples from 26 pitches in German shepherd dog were amplified and 23 variable markers were obtained by an AFLP method with 7 selected primers. The results of t test suggested that E39/T33-22 and E39/T38-35 had significant effects on trunk shape. The same did E39/T49-5 on limb shape, and E39/T38-16 on pregnancy rate. These Indicated that marks possibly was major gene or association with characters.3 The relationship between AFLP markers and body measurements as well as reproduction in RottweilerBlood DNA samples collected from 23 pitches in Rottweiler breed were amplified and 108 variable markers were gotten by an AFLP method with 7 selected primers. The results of / test suggested that E35/T32-73 had significant effects on body height. The same did E35/T32-1, E39/T32-31 on coax height, E39/T33-16, E39/T33-60 on shape of head and neck, E35/T32-39 and E35/T32-120 on trunk shape, E35/T32-109,E35/T38-16 and E39/T32-31 on limb shape, E35/T32-15,E35/T32-65 on litter size and survivor rate of pups. These Indicated that marks possibly was major gene or association with characters.