Preliminary Analyses Of Function For A ERF Transcription Factor-Os922 In Rice

Sugars play the key role in growth, development and metabolism of plant life. Moreover, multiple lines of evidence indicate that many of biotic and abiotic stress processes are also regulated in response to sugar. For example, sugars have been postulated to help regulate the synthesis of phytohormones, which were important signal molecules in response to biotic and abiotic stress, such as gibberellins and abscisic acid. Conversely, phytohormones, such as abscisic acid, gibberellins and cytokinins have been shown to help regulate sugar metabolism and/or transport. Similarly, sugars have been shown to regulate the expression of components of phytohormone response pathways and phytohormones regulate the expression of some genes encoding possible components of sugar-response pathways.The ERF(ethylene-responsive factor) belongs to ERF subfamily of AP2 family, which is unique to plants , and was identified as EREBP(ethylene-responsive element binding protein ) at first. Recently, it was approved that the expression of many ERF genes is induced by pathogen infection and environmental stress.A Os922 gene was cloned from cDNA of rice through PCR, which encodes a ERF transcription factors and transformed into rice (Oryza satinv cv Xiushui11) by Agrobacterium-mediated transformation. In transformation procedure, some factors in relation to transformation efficiency were studied systematically, involving light conditions during coculture, method of eliminating Agrobacterium and compositions of regeneration medium, and the promotive effect of light, especially, a 116 light/8h dark photoperiod was observed.It was confirmed that Os922 was integrated into rice genome by PCR and Southern blot techniques. The result, which the expression of Os922 enhanced the sensitivity to glucose and decreased in chlorophyll content in T1 progenies , suggested that Os922 perhaps play some roles in the sugar signal transduction .