Studies On The Characteristic Of High-Expression And Preparation Techniques Of The Phycobiliprotein In Spirulina Platensis Strain Sp-CH32

Spirulina platensis is multi-cell filamentous blue-green alga, simultaneously, it is an economic microalga with the largest scale of research and exploiture inland and abroad. The phycobiliproteins are kinds of active protein which extensively exists in spirulina, and it bears phycobilin with fluorescence idiosyncrasy, so the phycobiliproteins possesses great values in theory research as well as exploiture. The phycobiliproteins can not only be used as natural pigments in food, cosmetic and dye industries, but also be applied to the area of medicaments and healthy foods since it can improve immune capability and resist tumour, radiation, caducity, anaemia and so on. In addition, as fluorescence molecular probe and photosensitive reagent, it has fine prospect in clinical diagnoses, immunochemistry, bioengineering and tumor laser therapy.In order to screen out the excellent Spirulina platensis strains with high-yield phycocyanin in large-scale cultivation, The contents of photosynthetic pigments of seven strains, named Sp-S, Sp-T, Sp-YE15, Sp-CH32, Sp-HUF01, Sp-IDF and Sp-YC09, which have representation in taxology and exhibit eximious quality in large-scale production, were compared. The results indicated that the contents of chlorophyll and carotenoid in Sp-CH32 were as much as the other strains', whereas the content of phycobiliproteins achieve 29.994% of dry weight of cells, the content of phycobiliproteins in Sp-CH32 was 20.9% higher than that in Sp-S which is highest in the other six strains and 36.9% higher than the average value of the content of phycobiliproteins in seven strains. Furthermore, the contents of phycobiliproteins of six different batchs Sp-CH32 in large-scale cultivation, were between 29.872% and 35.821%, and their average content is 32.412%. The results indicated that Sp-CH32 has excellent characteristic of stable and high expression phycobiliproteins not only in laboratory but also in large-scale cultivation.On the basis of the former works and the characteristics of high-content phycobiliproteins and cells fragility in Sp-CH32, we established a preparation technology...