Study On The Fast Propagation In Vitro Of Cotinus Coggyria

American royal purple is a variety of Cotinus coggria Scop in the United States. It is wildly applied in park-green engineering, therefore, it has an extensive prospect. The purpose of this experiment is to find the best methods and conditions for fast peopagation of American royal purple, and to serve the industrialization of this good specie. The main results read are as follows:1.In the establishment of in vitro fast propagation process of Cotinus Coggyria, we found that HgCl₂ was the best disinfector and the stem with sprouts had the highest survival rate. Eight minites sterilization is best for the survival rate of Cotinus Coggyria explants.2.In the bud-inducing experiment of Cotinus Coggyria from in vitro sprouts, the concentration and combination of different hormones have a great influence on the result. 6-BA/NAA (0.1/0.02) is the best choice to induce cluster buds, which have more cluster buds but no calli. Temperature is another affecting factor. The best result can be obtained at 26²8℃, under which the average propagating coefficient is 6.40.3. On the browning of Cotinus Coggyria. The degree of browning varies according to the age, size of explants and the time the explants were taken. Explants came from winter and the old explants had low brown rate. The active carbon played a role in inhibiting browning.4. Researches on rooting technique. (1) 1/2ML+IBA (0.2) and 1/2ML+NAA (0.2) are the best media, with inducement root rate (IRR) of 88.9% and 98.5%, respectively. The average rooting number of each stem is 2.71 and 2.64, respectively. (2) There are more roots if the media containing 6g/L agar than those containing 5.5 g/L or 6.5g/L agar. The roots will reach maxium if the media containing 7g/L agar. (3) Low concerntration of activated carbon is propitious to root. Though the difference between the rates of rooting with 0.2g/L or 0.4g/L activated carbon is not significant, both of them are higher than other treatments. And the rates of rooting reach up to 100% with 0.4g/L activated carbon. (4) The ratio and the number of the in vitro sproutsincreased when the shoot is higher. Though the ratio and average number of sprout is low when the shoots are 21²5mm, the root length and ratio of surviving roots are hinghest. (5) The best temperature for rooting is 22 °C. (6) The concentration of sucrose from 15g/L to 30g/L is suited for rooting, while 15g/L or 25g/L is the best, in which the ratio of rooting is 96.7% and 92.2%, and the length of roots is 8.47cm and 7.86cm. The rate of rooting and the length of roots showed there no significant difference for these two concentrations, but both of them were higher than those in other concentrations. (7) The longer time of culture is propitious to rooting, but this will surely increase the cost. Therefore, considering the economical views, the best time for successive culture in bottle is 8¹0 weeks.5.In the process of cultivating the in vitro sprouts, the environment is important for the survival rate. The ideal environment is as follows: the light strength 3200Lx, RH 90¹00%, and the matrices is veimiculite. The root number and cultivated method (potted or outdoor) have little effects on the survival rate.6.In the transplanting research, the technology, environment have great effects on the survival rate. The highest servival rate was obtained when cultured with nourishment potted, half shade and covered with films. In the three experiments of different matrices, all the sprouts cultivated in nourishment pot have high survival rate and grow well. The experiment B (2 shares garden soil, 2 shares putrid leaves soil and 1 share sand) got the best result, the survival rate is 97.3% and the average height living around 40 days is 8.5 cm.