The Research For Rapid Propagation And Control-browning Of Phalaenopsis

In this study,Phalaenopsis culturing-plants induced by peduncle buds as explants from healthy and adulting plants were get in single and double factors'randomized absolutely experiment designs,the best culture mediums in different stages were selected; PLB were induced by young leaves from culturing-plants in single and double factors'randomized absolutely experiment designs and had grown into culturing-plants and best culture mediums in different stages were selected.The research which was on the leaves'control- browning were taken.The main results were as follows:1,The better culture mediums of inducing tufty buds in different stages are:(1)The buds which is in the middle are the best explants,germination rate is 43.33%。(2)The best culture medium which induces tufty buds is1/2MS+6-BA5.0mg/L+NAA0.5mg/L+ CM10%,the inducing rate is 80%,the browning rate is lower。(3)The best culture medium which proliferates tufty buds is the same as the inducing one,the proliferation is 2.89,the tufty buds are healty and green。(4)The best culture medium which is rooting and sronging is 1/2MS+6-BA3.0mg/L+NAA0.5 mg/L,the rooting rate is 100%,the averaging roots are 4.81 and the averaging leaves are 5.23。2,The best culture mediums of inducing PLB from leaves in different stages(1)The best culture medium which induces PLB is VW+6-BA5.0mg/L+CM10%,the inducing rate is 54.44%。PLB are big,full,healthy and propitious to proliferate。(2)The best culture medium which proliferates PLB is 1/2MS+6-BA3.0mg/L+NAA0.3mg/L+ Banana Juice200g/L,proliferation is 3.41.PLB proliferated rapidly and arranged lossenly,PLB's colour is green and surfaces are wet。(3)The best culture medium which differents PLB is 1/2MS+6-BA3.0mg/L+NAA0.4mg/L+ Banana Juice200g/L,the PLBS different trimly,rate of survival is improved.(4)The effects of other factors on proliferation:The liquid-culturing,incising in lamella,block in 10 PLBs are propitious to proliferation。3,The results of control-browning are as follows:(1)The darking pretreatment as long as 10 days can reduce browning rate to 48.60%。(2)Different kinds and concentration of Resist-brownings can reduce browning rate, 2g/LAC is the best one,browning rate is 35.80% and there is no bad influence to inducing PLB。(3)The heating pretreatment as long as 1 hour can reduce browning rate to 45.17%,the culturing materials are all right。...