Preliminary Studies On In Vitro Propagation Of 'Mopan' And 'Shang Xi Zao Sheng' Persimmon

Pilot studies were conducted on in vitro propagation of 'Mopan' and ' Shangxizaosheng' persimmon. The effect of culture establishment with buds or young shoots taken at different time was compared. Of test tube plantlets the effect of different kind of hormones and their proportion in subculture, phenol adsorbent and antioxidant on the prevention from browning, hormone and dark culture on the rooting were discussed. The main results were as follows:1. Necrosis of actively growing shoots tips was over 80%, which limited the culture establishment. Compared with shooting tips, buds taken in January to March or September to December were the much better explants, of which more than 80% survived and started growing in the first generation.2.The culture of Mopan persimmon could be established in medium with ZT 2mg/L or BA5.0~7.5mg/L. But plantlets cultured with BA in the first generation couldn't elongate and most of them grew weakly or even died in the subsequent subculture with BA. Adding Img/L BA and Img/L ZT to the modified MS medium could promote their growth and increase the rate of effective shoots and the rate of differentiation.3.Subculture in the medium with ZT were better than with BA. For 'Mopan' persimmon Img/L ZT could be used for long time subculture. 2mg/L ZT could promote proliferation of Shangxizaosheng persimmon. So did BA 5.0mg/L with NAAO.lmg/L, but the buds grew into form of rosette . Transiting them into the medium with Img/L ZT and 0.05mg/L IAA could promote their elongation. Alternate culturing with BA and then with ZT every other generation could be used to increase differentiating rate and reduce costs of test tube plantlets.4. For Mopan persimmon the basis of shoots dipped into 250mg/L IBA solution for 30s and then cultured in 1/2MS medium in darkness produced single thick roots, mean length of the roots was 54mm. For Shangxizaosheng persimmon, the basis of shoots dipped into 200mg/L IAA solution for 20min and then cultured in darkness for 12 days before resuming illumination, rooting rate was 20%, mean number of roots per shoot was 2.5. PVP could efficaciously prevented dormant bud from browning in the process of culture establishment and subculture.