Study On Porcine Oocytes Maturation In Vitro And Parthenogenetic Activiation

IVM,parthenogenetic activation and karyotype analysis were performed in porcine oocytes in this thesis.Development and karyotype of parthenogenetic embryo were mainly investigated. meanwhile,The situation of extrusion about the polar bodies from the porcine oocytes activated by electricity were observed in 7h ; Then development of oocytes that had the first or no polar bodies were compared; Effect of decreased saline temperature of preserving ovaries on development in vitro maturation were studied. The result of the experiment is described as following: 78.54% of oocytes extrude the second polar bodies after they were activated by electricity, and subsequently the rate of SPB in an hour dramatically decreased; Porcine oocytes were cultured in vitro for 0h (control), 3h, 4h, 5h and 6h after electrical activation , then they were treated in 6-DMAP/CHX ,there were no statistical different in the mean number of nuclei per blastocysts and cleavage rate (p>0.05).But blastocysts rate of the control group in 6-DMAP group was significantly higher than other groups (p<0.01). Blastocysts rate of the control group in CHX group was significantly higher than interval 5h, 6h groups (p<0.01); In the experiment about chemical treatments for 6h after electric activation in 4h intervals , there was no significant different for cleavage rate (p>0.05), but blastocysts rate of the control group was significantly higher than other groups (p<0.01). Blastocysts cultured in control almost were diploids, blastocysts in other groups mostly were haploids , and there were no significant different (p>0.05) . The result showed that the activation effect gradually declined when interval time become long; Blastocystes activated by electricity and 6-DMAP,CHX,CB+6-DMAP (with 4h interval between them) mostly were haploid. Rate of oocytes that extruded the SPB were very high for oocytes which had the first poplar bodies, conversely rate of oocytes extruded the first poplar bodies or SPB were very low for oocytes which had not the polar bidies after oocytes were maturated in vitro for 44h. Porcine oocytes were cultured in vitro for 0h (control), 3h, 4h, 5h and 6h after electrical activation , then they were treated in 6-DMAP/CHX . cleavage percentage of the a,b.c,d,e group in 6-DMAP group was higher than other groups (p<0.05)..Blastocysts percentage of the a group was significantly higher than other groups (p<0.05). there were no statistical different for cleavage rate in CHX group (p>0.05)Blastocysts percentage of the a group was higher than d,e,f,i,k,h groups (p<0.05); In the second study, there was no significant different for cleavage rate and blastocysts rate (p>0.05), The result showed that development of oocytes which had no poplar bodies were worse than that of oocytes which had the first polar bodies ;Oocytes which had not the polar bidies could extrude the bodies ,but development couldn`t be enhanced even though they were activated by chemical activated ;The first polar bodies are an important character of oocytes maturation. There were no significant different for IVM when saline temperature decreased from 37℃-38.5℃ to 31℃,32℃,33℃,34℃,36℃。...