Studies Related To Parthenogenetic Activation Of Porcine Oocytes Matured In Vitro

This thesis was designed to research factors affecting parthenogenetic activation of porcine oocytes matured in vitro .The text was arranged in two parts:the first part is a critical review of research in the field; the second part includes three sections: (1) effects of ethylenediaminetetraacetic acid, energy substrate, amino acids on in vitro maturation of porcine oocytes and in vitro development of porcine parthenogenetic embryos (section 1) ; (2) effects of EDTA-Na on in vitro maturation of porcine oocytes and in vitro development of porcine parthenogenetic embryos(section 2); (3) effects of the different chemical activation methods and electro-activation parameters on the vitro development of porcine parthenogenetic embryos (section 3), in order to improve the efficiency of parthenogenetic activation and nuclear transfer of porcine oocytes.Conclusion: (1) relatively high concentration of energy substrate is detrimental to in vitro maturation of porcine oocytes;(2) relatively high concentration of energy substrate +amino acids in culture medium is detrimental to the early development of porcine parthenogenetic embryos;(3) addition of 100μM EDTA-Na to maturation medium is the most beneficial to the early development of porcine parthenogenetic embryos in vitro;(4) addition of 100μM EDTA-Na to embryo culture medium is the most beneficial to the early development of porcine parthenogenetic embryos in vitro; (5) addition of 100μM EDTA-Na to maturation medium and culture medium is beneficial to the early development of porcine parthenogenetic embryos in vitro;(6) addition of different...