Characterization Of Several Enzymes Activity Variations In Wheat Resistant And Susceptible Varieties To Aphid

This research cleared the function of four enzymes (Phenylalanine ammonia-Lyase, Polyphenol Oxidase, peroxidase and catalase) in wheat resistance to Sitobion azvenae (Fabricius) . Five wheat, two high-resistant cultivars/selections (KOK1679 and My295), one middle-resistance cultivars (Li), and two susceptible cultivars (Beijing837 and Beijing411) were been researched. The Phenylalanine ammonia-Lyase, Polyphenol Oxidase and peroxidase activity of aphid-resistant wheat were higher than aphid-susceptible wheat, and after aphids infested, flag leaf and ear enzymes activities were increased. Catalase activity of KOK1679 and Li were higher than other wheat. After aphids feeding, resistant wheat activities changes of Phenylalanine ammonia-Lyase were more dramatic, while Polyphenol Oxidase, peroxidase of susceptible were more dramatic.This research cleared the function of four enzymes (Phenylalanine ammonia-Lyase, Polyphenol Oxidase, peroxidase and catalase) in wheat resistance to aphids. Five wheat, two high-resistant cultivars/selections (KOK1679 and My295), one middle-resistance cultivars (Li), and two susceptible cultivars (Beijing837 and Beijing411) were been researched.PAL activity of different cultivars and different growing stages were different. Activities of KOK1679 of seedling stage, shooting period and flag leaf were higher than other cultivars, and the lowest was Beijing837. Of all ears, activity of My295 was the highest, and the lowest was Beijing837.PPO activity of different cultivars and different growing stages were different.. Activities of KOK1679 of seedling stage and shooting period were higher than other cultivars, and the lowest was Beijing837 and Beijing411. Of flag leaf and ears, activity of Li and My295 was the highest, and the lowest was Beijing837.POD activity of different cultivars and different growing stages were different. Activities of KOK1679 and Li of seedling stage were higher than other cultivars, Activities of KOK1679 at shooting period was highest, and the lowest were Beijing837 and Beijing411. Of flag leaf, KOK1679 was the highest. Of ear, Beijing411 was the highest.CAT activity of different cultivars and different growing stages were different. Activities of KOK1679 of seedling stage were higher than other cultivars, Activities of Li, KOK1679 and My295 at shooting period were higher than other cultivars. Of flag leaf, KOK1679 was the highest. Of ear, Li was the highest.After infested by Sitobion azvenae (Fabricius) , Of flag leaves, the change of PAL of My 295 was most dramatic. PPO of Beijing411and Beijing 837 were most dramatic. POD of Beijing411 was most dramatic. CAT of KOK1679, Li and Beijing837 were increase, and others decrease.After infested by Sitobion azvenae ( Fabricius ), Of flag ears, the change of PAL of My 295 was most dramatic. PPO of Beijing411and Beijing 837 were most dramatic. POD of Beijing837 was most dramatic. CAT of KOK1679, My295 and Beijing837 were increase, and others decrease.After infested by Schizaphis gramminum, of 10aphids/wheat treatment, PPO activities of all cultivars at 72h were the highest. Of 20aphids/wheat treatment, PPO activities of KOK1679, My295, Li, Beijing837 and Beijing411 at 48h, 48h, 12h, 12h and 24h were the highest. Of lOaphids/wheat treatment, POD activities of all cultivars at 48h were the highest. Of 20aphids/wheat treatment, except My295, POD activities of all cultivars at 72h were the highest.Gels stained for PPO and POD identified differences in the isozyme profiles of different time after infested by aphids, and some of the profiles become darker according the prolonged intervals, some become thiner.