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Study On Nutritional Physiology And Effective Components Of Officinal Insect-Eupolyphage Sinensis Walker

Posted on:2006-10-14Degree:MasterType:Thesis
Country:ChinaCandidate:Q F TangFull Text:PDF
GTID:2133360152999378Subject:Agricultural Entomology and Pest Control
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1. The study on the hatchability of egg pouch and biological characteristics of nymphae of Eupolyphage sinensis Walker Based on the egg pouchs and nymphae of Eupolyphage sinensis, the hatch rate of eggpouchs was low, around 50%. Hatching climax of the egg pouchs was about 40-day afterthe egg pouchs were laid. E. sinensis Walker below the 3rd instar nymphae had the color ofwhite or weak yellow, its breast and abdomen had yellow spot, especially the back of footand abdomen, its alimentary canal had the color of black and brown after it had been fed. E.sinensis above the 3rd instar nymphae were like adult, ellipse, black and brown and theshine of purple. With the growth of E. sinensis, its color gradually was deepened. Thenymphae between the 1st instar and the 3rd instar growed slowly, however, the nymphaeabove the 4th instar growed quickly. Compared with the development time of other instarnymphae, the 1st instar nymphae's was 12.15d in average, which was very different fromother instar ones, but the one's of the nymphae above the 2nd instar was nearly the same,averaging 20d. The exuviated time of nymphae concentrated during 24﹕00 and 6﹕00(thenext day). A few nymphae exuviated during 12﹕00 and 24﹕00. It was clear that theexuviated time of nymphae concentrated at the later half-night. Different instar nymphaehad the different concentrated time to exuviate. The 1st instar,the 2nd instar and the4th~7th instar nymphae exuviated peak day were 2~4d,4~6d and 2~4d, respectively ,afterthe exuviations had been first seen.2. The influence of breeding dust on the Eupolyphage sinensis Walker In order to study the effect of different breeding dusts on the growth and developmentof E. sinensis Walker, compost, mushroom culture and garden dust that were chosen as therepresentative man-made breedingdust were breed. It was observed that E. sinensis Walkerhad tropism to different breeding dusts. The results showed that organic content: compost>mushroom culture>wildness inhabiting dust>garden dust, proportion: garden dust>mushroom culture>wildness inhabiting dust>compost, pH: wildness inhabiting dust>compost>garden dust>mushroom culture. Compost was superior to mushroom cultureand garden dust in the growth and development of E. sinensis Walker, but E. sinensisWalker tended towards inhabiting the mushroom culture. The spatial distribution pattern ofE. sinensis Walker in rearing pool were studied by comparison of Variance-Menu Ratiowith indices of aggressive intensity (dispersal index, clumping index, mean crowding,patch index and intensity index). The results indicated that the spatial patterns of E.sinensis were clumped in rearing pool and they were fond of the margin of rearing pool.When the density increased, they dispersed rearing pool.3. The study on the different feeds and nutritional physiology of Eupolyphagesinensis Walker Based on the anatomy of the low instar nymphae digestive tract structure, the resultsindicated that the digestive tract of low instar nymphae of E. sinensis was similar to that ofadult. The low instar nymphae had gastric caeca and Malpighian tubles. The low instar hadassimilation ability by scaling the ratio of different part of digestive tract. The 1st instarnymphae could be fed with humus and manpower commixing feed by observed theremains of digestive tract. The newly born nymphae needed feed to supplement itsnutrition. In this paper, the low and high instar nymphae were randomly divided into fourgroups, respectively. The stuff of Feed in each group were A(wheat: corn: soybean=6:3:1);B(greengrocery: corn: soybean=6:3:1); C(wheat) and D(greengrocery) respectively. Thisexperiment reviewed diversities from relative growth rate (RGR), relative consumptionindex (RCI), approximate digestibility (AD), efficiency of conversion of digested (ECD)and efficiency of conversion of ingested (ECI) via feeding E. sinensis Walker. The databaseshowed AD and RCI were decreasing progressively in D>C>B>A at low instar. ECD,ECI and RGR to A were the highest. But RGR to A was not notably different with one to C.At high inst...
Keywords/Search Tags:Eupolyphaga sinensis Walker, Nutritional physiology, Supercritical CO2 fluid extraction, Effective components, Pharmacological study
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