Studies On Chemical Induction Of Flax Polyploidy

Flax seeds, Fanny, were taken as the experiment materials, and then chemical induction, tissue culture and transplanting were studied. So polyploidy plants were gained and were made to get small-scale reproduction. The main results were as follows.1. On the base of experiments of quadratic regressive analysis after single factor experiment, induced survival rate was affected mainly by the concentration of colchicines solution, secondly by worked time, and relatively little by induced temperature and soaked time with pure water. The results showed that the best induced effect could be obtained under the conditions as follows: immersion by 16 hours with water, temperature 20℃, colchicines concentration 0.16%, worked time by 12 hours, the induced survivor by 24.67%.2. The budding flax seeds induced by colchicines solution grew on the different pH and culture medium, and were cultivated on the basal medium supplemented with various plant growth regulators during rooting phases. The effects of pH, different culture medium and plant growth regulators on root induction of mutation plantlets were studied. The results showed treatment of NAA(0.1~0.2mg/L) or IBA(0.3mg/L) or the combination of IBA(0.1mg/L) and NAA(0.3mg/L) on the 1/2B5 medium favored obviously rooting. The above substances promoted rooting ratio (above 80%).3. Flax plantlets of morphological variation were taken as experimental materials. The effect of domesticated time, temperature, different number of roots, transplant matrix, green house and outdoor on transplant survival percent of plantlets of morphological variation were systematically studied. The results showed the transplant survival percent was the highest (above 90%) when we selected seedlings with 6~8 roots, domesticated seven days in 25℃ in culture house, took the loess and sand and coking chaff (5:3:2) as matrix, cultivated ten days in green house, and then six days in open air, and finally transplanted in farmland. A set of simple available, practical and efficient technique of transplant plantlets was obtained.