Study On Fungicidal And Herbicidal Components Of Metabolites Of Botrytis Cinerea BC1 Isolate

The Botrytis cinerea BC1 isolate from Tagetes patula has been studied on the fubgicidal, herbicidal activity and cultural condition, isolation and identification of herbicidal components in this paper. The main results are as follows: 1. Using the test of uni-factor and orthogonal design, inoculum's volume was 8 entries per 100mL medium, and the better condition to produce herbicidal components from the BC1 isolate is to culture it in Fries medium with pH7 at 24℃under dark and static condition for 20 days. 2. The fungicidal activity of culture filtrate of the Botrytis cinerea BC1 isolate had been bioassayed. The results showed that inhibiting rate against Amaranthus retroflerus, Cochliobolus sativus,　and　Caeumannomyces　graminis were up to 60%. In whole plants tests, and spraying wheat plants before disease development with a culture broth provided protective control of up to 40% against disease caused by Rizoctonia cerealis and Cochliobolus sativus at the concentration of 50 ml·L-1, and culture filtrate of Botrytis cinerea BC1 isolate showed a curative effect of up to 30% against disease caused by Rizoctonia cerealis and Gaeumannomyces graminis at the concentration of 500 ml·L-1. 3. The herbicidal activity of culture filtrate of Botrytis cinerea BC1 isolate had been bioassayed. The results showed that inhibiting rate against Amaranthus retroflerus , Setaria viridis, Digitaria sanguinali, and Echinochloa crusgalii were up to 60%. In whole plants tests, at the concentration of 150 ml·L-1, inhibiting rate against Amaranthus retroflerus was 83.4% in soil treatment , and inhibiting rate against Setaria viridis was not up to 60% in soil treatment. 4. After repeatedly open column chromatography, compound 1# was obtained from of culture filtrate of Botrytis cinerea BC1 isolate from Tagetes patula for the first time with bioactivity-guided fractionated. Comparing with the standard spectra of chromatography chart such as UV, IR , MS and 1H NMR, compound 1# was primarily identitied as s -(+)-abscisic acide(common name is ABA). The results indicated that the values of EC50 of compound 1# was 0.019 mg·L-1, against germination of Amaranthus retroflexus.