Study On Tissue Culture Of Dendrobium Huoshanense

The officinal values was introduced and present situation of Dendrobium tissue culture in vitro and rapid propagation in the world was generaliced. On this basis, Stem tissue culture in vitro and rapid propagation of rare and endangered Dendrobium huoshanensn were studied in this paper.The results showed that the optimal initial medium for stem culture and rapid propagation of Dendrobium huoshanensn was MS+6-BA 2.0 mg.l^-1+NAA0.5 mg.l^-1+ Sugar2%, the optimal proliferation medium for adventitious buds was MS + 6-BA1.0 mg.l^-1+ NAA0.1 mg.l^-1 +Sugar 2%, whose multiplication mediums was 5.11. Adventitious buds were grown at (20±l)℃ under fluorescent lights(light intensity 20001x). The optimal multiplication mediums, induced medium and rooted medium of protocorm were respectively N₆ + KT2.0 mg.l^-1+NAA0.1 mg.l^-1+Sugar2% , N₆ +6-BA1.0 mg.l^-1+NAA0.1 mg.l^-1+ sugar 2%, 1/2MS + IBA 0.5 mg.l^-1+ Sugar 2%. The best transplanting technique was also obtained, which was taking "small bark + small stone + coconut shell powder ( 1.5:1.5:1 ) "as matrix, overshadowing 70% and transplanting in March , April or October. The transplanting survival percent was cver 80% using this technique.A set of whole technique of Dendrobium huoshanensn tissue culture in vitro and yapid propagation wa obtained in this paper.