The Analysis Of Genetic Diversity Among Some Buffalograss Materials Determined By RAPD Markers

With the progress of turf projects, demand of water resource increase yearly in China.However,water resource in China is under average level If the would,for this reason,it is feasible to empolder water-saving and drought-endurable turf. Buffalograss Buchloe dactyloides is a warm season turfgrass which is excellent of drought hardiness.This article analysis 10 buffalograss material by RAPD, is for the purpose of applying RAPD technology constructs DNA Fingerprint, atlas; Based on RAPD analysis data discussion plant's blood relationship relations, through to special fragment screening appraisal, will obtain the special mark to lay the foundation for the next selecting and breeding improved variety.10 Buffalograss materials were analyzed by Random Amplified Polymorphic DNA (RAPD) markers to determine their genetic relationship. Of the 260 random nucleotide primers screened, 14 showed steady banding patterns and produced 129 bands with 72 polymorphisms.Using highly effective primers the OPE21 and OPN10 Constructs The DNA fingerprint atlas, according to The band of primers OPE21 and OPN10 calculates 10 materials similarity modulus scope is 0.832~0.958.Using Un-Weighted pair-Group Method with arithmetic means(Abbreviation UPGMA) constructs the phylogeny tree and acts according to the phylogeny tree, according to UPGMA gathers cluster, according to the gathers, the analysis result indicate, The 10 buffalo grasses materials obvious division two big class groups, Respectively is female plant three, four and five gets together and other plants in together. Explained these two branches the buffalograss materials are have the near blood relationship relations respectively, between them has the similar heredity background.Obtains three special mark bands with good duplication and strong signal, OPE21-697, OPN10-1543 and OPN10-1000 may take special mark bands for male NO.2 (8), female NO.4 (4), and monoecism (7) separately.