Study On Different Susceptibility To Fatty Liver And Expression Of Lipoprotein Lipase In Goose

Sichuan white geese and Landes geese were studied in this experiment. The lipoprotein lipase (LPL) expression of adipose tissue and muscle were analyzed by RT-PCR method. The effect of high-fat diet feeding and high energic carbohydrate force-feeding on fat deposition of geese were analyzed, and the genetic difference, along with its mechanism, between the two breeds geese in the susceptibility to fatty liver in response to over-feeding were discussed. These experimental results showed:The LPL expression abundance in subcutaneous adipose tissue, abdominal adipose tissue, pectoral muscle and thigh muscle of the two breeds geese fed a high-fat diet containing 10% lard were markedly lower than the control geese, and the post-heparin LPL activity in plasma also markedly decreased ( Landes geese: 2.97 ± 0.58 palmitate/ml.h VS 4.98 ± 0.72 palmitate/ml.h; Sichuan white geese: 2.78 ± 0.47 palmitate/ml.h VS 4.75 ± 0.63 palmitate/ml.h ). This indicated that the decrease in LPL activity limited the development of adipose tissue and muscle to a certain extent.The plasma VLDL and TG concentration were markedly higher than control geese in Landes geese fed a high-fat diet, the fat deposition into subcutaneous adipose tissue and abdominal adipose tissue depended on hepatic VLDL-TG secretion. While the plasma VLDL and TG concentration were not markedly changed in Sichuan white geese fed a high-fat diet, it was likely that the fat from the high-fat diet deposited directly into adipose tissue in this breed.The ratio of liver to body weight and the percentage of liver lipid in Landes geese overfed a diet rich in carbohydrate were markedly higher than Sichuan white geese overfedthe same diet (ratio of liver to body weight: 8.55±1.75% VS 6.07±l .46?/o; the percentage of liver lipid: 46.87 + 7.39% VS 38.47 + 7.16%). Overfeeding resulted in a markedly increase in plasma VLDL and TG concentration of the two breeds goose. But it was markedly higher in Sichuan white geese than in Landes geese (P<0.05) , so did the LPL activity(11.40 + 0.94 VS 7.61 +0.87)(PO.001). This showed that the efficiency exported to the extrahepatic tissue of lipids synthesized in liver and the hydrolyzing efficiency of TG catalyzed by LPL were higher in Sichuan white geese than in Landes geese, which was the possible cause that Sichuan white geese was less susceptible to fatty liver than Landes geese but the development rate of subcutaneous adipose tissue and abdominal adipose tissue was higher than Landes geese.After overfeeding, Sichuan white geese showed that the ratio of liver to body weight was positive correlation to body weight ( r=0.57,p<0.05 ) , TG concentration(r=0.68,p<0.05) and VLDL concentration (r=0.55,p<0.05) respectively. There was a better balance between hepatic lipogenesis and lipoprotein exportation to extrahepatic tissue in Sichuan white geese. However similar correlations were not founded in Landes geese, the ratio of liver to body weight was negative correlation to LPL activity(r=-0.54,p<0.05) , the lipids preferentially stored into liver in Landes geese.