The Study Of Isolation And Identification Of Gomphus Purpuraceus

Gomphus purpuraceus is a kind of macro-ectomy-corrhiza emerging in forests in Summer and Autumn every year, and is of the important value in development and utilizatin. For the rare genetic resource of reservation available and exploratin of development, the studies on ecological conditions, nutrilive components, isolation and identification were conducted and the results is as following as:1. Analysis of the nutritive components of Gomphus purpuraceus:It was analysed on dry weight basis, the crude protein (N X 6.25) content of G purpuraceus fruitbody was 26.14%, the content of 17 amino acids determined in G purpuraceus fruitbody protein was 17.92%, and the total essential amino acids content of the all amino acids was 47.6%; and G. purpuraceus fruitbody contained many kinds of mineral nutrients and vitamins, such as K, Na, Ca, Mg, P, Fe, Co Mn, Zn, Se, vitamin B1 vitamin B2 etc..Among all these chemical components, the contents of Fe, Zn, Se, vitamin B1 and vitamin B2 were especial high.2. Studies on the ecology of Gomphus purpuraceus:The distribution of G purpuraceus in Xiling Gorge of Three Gorges area and the environmental factors of G purpuraceus habitat, such as topography, elevation, vegetation, meteorology, soil conditions etc. were studied, and the main environmental conditions of G purpuraceus habit were clarified. The results showed that Gomphus purpuraceus is a kind of rare ectotrophic mycorrhizal fungi with higher nutritional value.3. Isolation and identfication of Complins purpuraceus:The tissue isolation from different parts of different developmental phases of fruit bydy and mycorrhiza was made with 5 media. The results showed that mycelia from fruit body grew very slowly and thickly, mycelia from mycorrhiza and rhizomorph grew quickly and thinly. Out of 5 media, medium D was optimal medium for tissue isolation. The isolates from different resources were appraised through DNA fingerprinting in comparison with that of dry basidiocarps of Gomphus purpuraceus, and RAPD patterns were sharp and stable by 8 random primers. The results suggested that isolates from fruit body had the same DNA fingerprinting patterns as that of the basidiocarps tissues (such as pileus and stipe), whose similarity coeffecient was 0.884. However, the RAPD patterns of the isolates from mycorrhiza and rhizomorph were different from the others (including the basidicarps tissues). These results further revealed that some isolates from fruit body were real isolates of Gomphus prupruaceus and the isolates from mycorrhiza and rhizomorph were not real isolates of the Gomphus purpuraceus.