Establishment Of High Efficient Plant System And Genetic Transformation Of COI1 Homolog Gene In Tomato

Tomato (Lycopersicon esculentum Mill.), which is a kind of annual herbage and belongs to the Solanaceae family in the plant kindom. The fruits contain plentiful nutrient contents and richer sweet juice.It is one of the most important vegetables and one of the model plants which are most studied in the biology. However, the present problem that the germplasm badly degenerates, the output and resistance falls, and the quality breaks up results from the effect of the diseases , insect pest, drought and chilliness in the course of the growth. Recently, more and more technics of molecular and cell biology including high efficient plant regeneration, plant genetic transformation, have been used in the studies of improvement of the quality and disease resistance in tomato.The tomato CY-1 and CY-2 were used as experimental materials. To establish a reliable, rapid and effective regeneration system in vitro and to carry the tomato genetic transformation out successfully, the factors affecting adventitious shoots regeneration were studied. The results indicated that tomato axenic seedlings grew rapidly and tidily after seeds are soaked in water and cultivated in dark .The frequencies and amounts of shoot organogenesis were significantly affected by the hormone concentrations for a given genotype. Compared with Zt, 6-BA had a better effect on inducing shoot regeneration. The regeneration frequency of cultivar CY-1 , CY-2 reached above the level of 95% in MS + 2.0 mg/L 6-BA + 0.02 mg/L IAA. The average numbers of shoots regeneration were 4 per leaf disc. The regeneration of the leaf disc was better than that of hypocotyl in the same genotype and the 9-day-old leaf had higher regeneration ability. While using the rooting medium of MS+0.1 mg/L BA +0.3 mg/L IAA, the rooting rate achieved nearly 75 %.COI1gene encodes an F-box protein to assemble SCFCOI1 complexes that are essential for responses to jasmonate (JA). Null mutations in COI1 abolish all the JA responses and exhibit resistance to JA-inhibition of plant growth, defect in pollen...