Studies On Regeneration System Of Apricot

From the study of plant growth regulators and their combination, base media, darkening treatment and other factors which affect on the stem subculture and proliferation, callus inducing, regenerating and rooting, we developed a regeneration system of hypocotyls, cotyledon and leaf which from Armeniaca vulgaris var. ansu. Adventitious plants regenerated from mature hypocotyls of Armeniaca vulgaris var. ansu have been got first time, with the rate of shoots regenerated being 37.8% and callus induced being 100% when cultured on the half NH4NO3 of MS medium supplemented with TDZ 2.0mg/L and NAA 0.5mg/L for 15d in dark and then exposed to light. A relative higher callus inducing rate(96.4%) and regeneration rate(28.6%) were also get when 6-BA 1.0mg/L and NAA 0.5mg/L was used. TDZ was better than 6-BA in plant regeneration. The effect of basal medium was modified MS> QL>WPM. The rooting rate was 75.8% when the buds cultured on half NH4+of MS+IBA 0.2mg/L ≧0.5cm shoot rates and proliferation multiples were the highest when the adventitious shoots were cultured on QL+6-BA 0.8㎎/L+IBA 0.1㎎/L. Rooting rate was 75.8% and average root number was 3 on modified MS+ IBA 0.2mg/L. Finally, plantlets were got. Adventitious shoots were found in 17～40d after 15～20d in dark on the regeneration medium. The highest callus rate(81.0%) and regeneration rate(21.5%) were got on QL+TDZ 2.0 ㎎/L+NAA 0.75 ㎎/L+AgNO3 4mg/L medium. TDZ was better than 6-BA to induce callus. AgNO3 could improve the inducing of callus. Callus were found mostly on the cut and the medium-touching side. Subcultures were useless to improve callus inducing and regeneration. ≧0 .5cm shoot rates and proliferation multiples of Armeniaca vulgaris var. ansu. were high on QL+6-BA 0.8mg/L+IBA 0.1mg/L as 24.2% and 5.3. The ≧0.5cm shoot rates and proliferation multiples of four cultuvars on C4 medium were Armeniaca vulgaris var. ansu.> Longwangmao> Caoxin> Lanzhoudajiexin. The callus were found at the petiole and vein of leaves after 10 d in dark, and adventitious shoots were found after 40～60d exposured to light. Depended on the result of the test on the QL+TDZ (0.5,1.0,2.0,4.0mg/L)+ NAA (0.25,0.75,1.25mg/L), TDZ was important than NAA for regeneration and callus inducing. In general, after 15d in dark, QL+TDZ 2mg/L+ NAA 0.75mg/L+ AgN03 4.0mg/L was better for callus inducing rate and regeneration rate, which were 100% and 8.3% respectively. The best result was obtained when the leaf adaxial side was touching the culture medium. In this position the regeneration obtained was 34.1%, which was higher than when the leaf abaxial side was in contact with the medium.