The Effects Of Microbial Inoculants On The Fermentation Process And Quality Of Corn Silage

This study evaluated the effects of microbial inoculants on the fermentation quality, microbial changes of silage, fermentation products, rumen degradation, aerobic stability and the productivity of the livestock respectively using the methods of laboratory silos, microbiology techniques, chemical composition analysis , in situ techniques and cow feed tests. The comparative study of microbial inoculants and other silages additives was also been done.In Expt.1, that the effects of microbial inoculants and formic acid on the pH, the number of LAB, yeast and mold, and the formation of lactic acid was evaluated during the different periods of corn silage (after day0.5, 1,2, 3,5, 7, 10, 20, 40 and 60 of ensiling) using the methods of laboratory silos and microbiology techniques. The results showed that the application of inoculants did not influence the number of LAB and yeast and the formation of lactic acid during the different periods after corn ensiling but increased the number of mold during the early stages of the silage. This maybe attribute to the number of the inoculated Lactobacillus, which failed to become the predominant bacteria during the fermentation process. The addition of formic acid inhibitted the growth of LAB at the early stage of the fermentation, decreased the production of lactic acid and the pH of the early stage of the silage and increased initially the growth of the yeast and mold but inhibitted the growth of mold after 5 days of ensiling.In Expt. 2, whole-plant corn was ensiled with the single or multiplex addition of Lactobacillus plantarum, Pediococcus pentosaceus and cellulose enzyme complex to evaluate the effects of LAB and cellulose enzyme complex on fermentation quality on the fermentation process, fermentation production and rumen degradeation (after 60 day of ensiling). There were 5 groups in this expriment, whole-corn were treated with additional of single Lactobacillus plantarum, single Pediococcus pentosaceus, Lactobacillus plantarum plus Pediococcus pentosaceus, Lactobacillus plantarum plus Pediococcus pentosaceus plus cellulose enzyme complex respectively, and silage with no additives served as a control. The results showed as following: The addition of single Lactobacillus plantarum increased the product of the acetic acid and the total organic acid and decreased the ratio of lactic to acetic acid. The addition of single Pediococcus pentosaceus decreased the product of the acetic acid and the total organic acid and enhanced the ratio of lactic to acetic acid. Both delayed the drop rate of the silage pH. Either the addition of single Lactobacillus plantarum or the addition of plus enhanced the concentration of NH₃-N and hence increased the ratio of the NH₃-N to TN. The addition of compound of the Lactobacillus plantarum, Pediococcus pentosaceus and cellulose enzyme complex to com silage decreased the NDF and ADF of the silage by 15.8% and 15.0% respectively, enhanced the 24h DM in situ degradation rates, showed no effect on the 48h DM in situ degradation rates and decreased the 24h and 48h NDF and ADF rumen degradation rate (p<0.05). The addition of single Lactobacillus plantarum, Pediococcus pentosaceus or Lactobacillus plantarum plus Pediococcus pentosaceus exhibited no significant effect on the concentration of the NDF and ADF and 24h and 48h DM, NDF...