Isolation And Screening Of Lactic Acid Bacteria From Corn And Corn Silage And The Effect Of Adding It On The Fermentation Quality And Aerobic Stability Of Corn Silage

The object is that selected epipHytic Lactic Acid Bacteria from corn and corn silage, Isolated,16S rDNA identified, acid production and growth curve analysis and then selected the Lactic Acid Bacteria, which suited for the corn fermentation, and apply it as inoculants, investigated the effect on fermentation characteristics and aerobic stability. It is a valuable study for development Lactic Acid Bacteria to provide theoretical foundation.16Lactic Acid Bacteria strains were isolated, after16S rDNA identified found that MRS-17is Enterococcus sp., MRS-35is Weissella sp., MRS-39is Lactococcus garvieae, MRS-01,MRS-06and MRS-11are Lactococcus lactis, MRS-04.MRS-09and MRS-10are Lactobacillus brevis.MRS-04, MRS-09and MRS-10are Lactobacillus brevis, MRS-20and MRS-31Lactobacillus casei. After acid production and growth curve analysis selected that homafermentative Lactic Acid Bacteria MRS-20and MRS-28, heterofermentative Lactic Acid Bacteria MRS-03and MRS-10suited for the corn fermentation.Use the selected Lactic Acid Bacteria strains MRS-20(Lactobacillus plantαrum), MRS-03(Lactobαcillus buchneri) and MRS-10(Lαctobαcillus brevis) as the additives to the ripening stage corn investigated the effect on fermentation characteristics and aerobic stability. There were eight treatments as followed:the control(no additives, CK), MRS-20at1.0X106cfu/g and1.5X106cfu/g of fresh forage, MRS-03or MRS-10alone at1.0X105cfu/g of fresh forage, mixture of MRS-03plus MRS-20(9:1), MRS-10plus MRS-20(9:1), and MRS-03plus MRS-10plus MRS-20at1.0×105cfu/g of fresh forage, respective. After1.3.5,7.15,30.45,60and90d of ensilage, assess the fermentation quality, respective and assess the aerobic stability of90d of ensilage.MRS-20can quickly reduce silage pH value at the early ferment, but at the end of the fermentation the silage the pH value, CP, NDF and ADF contents were no significant to the control(P>0.05). However MRS-20treatment can increase residual water-soluble carbohydrate concentrations at the end of ensilage, and MRS-20at1.5X106cfu/g of fresh forage is better than at1.0X106cfu/g(P<0.05). MRS-03or MRS-10alone and mixture of MRS-03plus MRS-20, MRS-10plus MRS-20.and MRS-03plus MRS-10plus MRS-20were not affect on the CP, NDF and ADF contents of the silages(P>0.05), but can increase residual water-soluble carbohydrate concentrations at the end of ensilage(P<0.05), except the additives MRS-10and MRS-10plus MRS-20.Additives can increased NH3-N contents, but not effect on the fermentation quality of corn silages. When applied MRS-10, the NH3-N content was highest than all of the others(P<0.05), and MRS-03plus MRS-20and MRS-03plus MRS-10plus MRS-20were had the lower NH3-N content(P <0.05), except the control. MRS-20can increase the lactic acid content of the corn silages, but reduce the content of acetic acid content and aerobic stability of the corn silages(P<0.05). MRS-03and MRS-10reduced the content of lactic acid, but increased the content of acetic acid content and aerobic stability of the corn silages(P<0.05). Mixture of MRS-03plus MRS-2O,MRS-10plus MRS-20, and MRS-03plus MRS-10plus MRS-20were not effect on the lactic acid content, but increased the content of acetic acid content and aerobic stability(P<0.05).