Research Of The Rice Heterosis Molecular Mechanism With The Functional Genomic Technology

Heterosis has been widely used in agriculture, and the analysis of its molecular mechanism is always the hot question. In this paper, the rice heterosis molecular mechanism and the P/TGMS gene were studied by the method of functional genomics combined with genetic analysis, with parent lines (PeiAi64s and 9311) of LiangYouPeiJiu and its F2 population as research materials. The main results are as followings:1 Molecular mechanism analysis of the rice heterosis by functional genomics method.1.1 In five gene chips, 845 genes that are discrepantly expressed were found.These genes are related to many plant functions, indicating that the heterosis was related with many metabolization pathways.1.2 The quantity of discrepant genes showed that the genetic discrepancy was notalways the same with the discrepancy of expressed genes, indicating that small-scale genetic discrepancy maybe result in large-scale genes discrepantly expression; in other way, some genetic discrepancies might not embodied on the discrepant genes in the experiment.1.3 Nine and eight discrepant genes were found respectively in the superior bulkand minor bulk in the dominant level. The results of our studies showed that the convergence of the discrepant genes in the dominant level were not related with the heterosis.2 Functional genomic study of the P/TGMS gene2.1 Twenty-four same discrepant genes were found by gene chip in the discrepant genes both between sterile and fertile parents, and between bulks of sterile and fertile F2 population.2.2 Five same discrepant proteins were found between the discrepant proteinsboth between sterile and fertile parents, and between bulks of sterile and fertile F2 population by proteomic technology.