| The mechanism of flowering is increasingly becoming the focus of botanic developemental biology research. In order to acquire a transgenic flower, whose anthesis is regulable ahead of time, CFL (from Cucumber) and two MADS-box genes (from Bamboo) were introduced into Sinningia speciosa respectively by Agrobacterium tumefaciens EHA105. In order to develop an efficient procedure of Agrobacterium mediated transformation, we optimized several parameters to achieve higher transformation efficiency. High efficient system of plant regeneration has been development. The results obtained are as following:1. Using recombination techniques, the gene fragment CFL, derived from pBluscriptSKII, was isolated and placed into pBI121, pCAMBIA13011 and pER8 vectors respectively, under the control of CaMV 35S promoter. CFL gene was transformed into leaves of Sinningia speciosa mediated by Agrobacterium tumefaciens EHA105. After twice selections of resistent cultures, transgenic plants with CFL regenerated from selective medium, and some transgenic plants were found to be positive by PCR analysis. Southern blot analysis revealed that the exogenous gene has been integrated into the plant genome. By now, some transgenic plants have already been abloom. Further functional analysis for CFL is ongoing.2. By means of RACE, two MADS-box genes are cloned from Phyllostachys praecox and both of they are placed into pCAMBIA13011 and pER8 vectors, and then transformed into Sinningia speciosa mediated by Agrobacterium tumefaciens EHA105. Transgenic plants with these two MADS-box genes regenerated from resistant explants (leaves) were obtained using hygromycin selective medium, and some transgenic plants were found to be positive by PCR analysis. Southern blotting also confirmed that the MADS-box genes have been integrated into the chromosomal DNA of these transgenic plants. The gene function is awaiting analysis.3. Different protocols were used in order to evaluate their effects on transformation...
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