Study On In Vitro Flowering Technology Of Three Species Of Herbaceous Flowers

For a long time, the ornamental forms of most indoor herbaceous flowers are mainly potted and cut flowers, but due to the limit of florescence, the ornamental time in a year is restricted to their flowering period. Studying the techniques of in vitro flowering can make flower buds in vitro differentiating, and flowers blooming in any season in test tubes. It is practicaly significant for developing new and different visual ornamental effect of flowers, and enriching the types of commercial flower production.In the study,3 kinds of popular potted flowers:Sinningia speciosa, Dendrobium nobile, Kalanchoe blossfeldiana’Red Sun’were used to conduct in vitro flowering test. The main influencing factors of strengthening seedling, in vitro flower bud differentiation, extending the flowering period were explored considering of technology for flower production. The appropriate culture medium and conditions were selected. The research provided tissue culture technology theory for commercial tube-flower production.The main results were as follows:(1)The medium MS with IBA 0.5mg/L and NAA 0.1mg/L was suitable for strengthening seedling of S. speciosa. Plantlets strengthened and rooted well and the leaf extended on the medium with 2g/L activated carbon added.WPM medium with 6-BA 0.3mg/L, NAA 0.2mg/L, sugar 40g/L and agar 9g/L was suitable for inducing in vitro flower buds differentiation of S. speciosa. The plantlets that seedling diameter greater than 3cm could be effectively induced flower buds.(2)1/2MS medium containing 50%KNO3,50%KH2PO4, supplemented with TDZ 0.2mg/L, PP333 2.0mg/L and sugar 50g/L was suitable for inducing in vitro flower buds differentiation of Dendrobium nobile ’Huoniao’ and D. nobile’T-O’. The plantlets with 2 section of shoot stem could be effectively induced flower buds.The medium MS containing NH4NO3, KH2PO4, KNO3, supplemented with suger 40g/L and ager 5g/L was suitable for extending the flowering period of D. nobile’Huoniao’. Browning phenomenon could be effectively overcome with 2 or 4g/L activated carbon added. While the flowering period was not shortened.(3)1/2MS medium with NAA 0. lmg/L, IBA 0.3mg/L, CCC 2.0mg/L and 2g/L activated carbon was suitable for strengthening seedling of Kalanchoe blossfeldiana’Red Sun’. Plantlets got thicker stem and blade with lg/L lactoprotein hydrolysate or 100g/L mashed potatoes added.MS medium with NAA 0.1mg/L, IAA 0.1mg/L,6-BA1.0mg/L and sugar 60g/L was suitable for inducing in vitro flower buds differentiation of Kalanchoe blossfeldiana ’Red Sun’. The plantlets that strengthening seedling cultured could be effectively induced flower buds.