| In the present study 5 varieties of black poplar and Populus.alba×P.Beolinensis were inoculated with Cytospora chrysosperma (Pers.) Fr. to investigate the disease resistance to Canker in poplar. The markers linked to resistance Canker in poplars were identified by techniques of RAPD markers and BSA (bulked segregation analysis).1. The susceptibility and resistant strains was tested in both the greenhouse and the field inoculation. 3 varieties of poplars (Populus deltoides×P.cathayana, Populus×xiaohei14 (the greenhouse), Populus.albaxP.Beolinensis) that resistant to Canker, and 4 varietie of poplars (Populus deltoidesx, Populus pseudo-simonii Kitag×P.nigra L., Populusxxiaoheil4 (the field), Populus×xiaohei2000) that susceptible to Canker were identified through the investigation of the disease resistance to Canker. By using BSA, resistance bulked and susceptible bulks were obtained.2. The best amplification reaction system for RAPD was done with 20μ1 of master mix, that consisted of 20ng of DNA template, primer 5μmol/L, dNTP20mM, TaqDNA1.5U, Mg2+2.5mM. The reaction mixture (containing the template DNA) was heated at 94°C for 2 min and then subjected to 30 cycles consisting of 30 s at 94°C, 30 s at 35°C, 1.5 min at 72°C, and a final step at 72°C for 7 min.3. 400 10-mer primers were evaluated in two replications using as template DNA samples from both R and S bulks. 16 primers, corresponding to a total of 108 fragments, displayed distinct amplification profiles with putative polymorphisms between R and S bulks. An average of 6.75 fragments per selected primer was observed. 52(49.1%) monomorphic bands, an average of 3.25 bands per selected primer, 56(51.9%) polymorphic fragments, an average of 3.5 fragments, was observed. The size of the amplification products range from approximately 0.3kb2.0kb.4. By using RAPD markers, in combination with bulked segregation analysis (BSA), SBSS12-1500, SBSS12-1250, SBSAN17-850, SBSAN17-390 were found linked to resistance Canker in poplars.
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