The Superovulated Distinct Effect Of Goats And Sheeps And Its Mechanism Research

Carried on the in great quantities superovulation experiment to the goats and sheeps at the Hebei cow sheep embryo center of "15" periods, The superovulated effect (the number of corpus luteum, the number of gained embryos and the number of viable embryos) of the goat experimental group which was markedly higher than the sheep experimental group.This research will study to investigate its mechanism in the variety regulation of the NO, FSH, LH, E2, P4 from the superovulated individual blood serum in order to raise the superovulated excellent effect and provide the theory basis. The choice better body situation and the age body weight more consistent Guanzhong -hybridised goats and Small tail han sheeps each 20 were the experimental animal, all divided into two groups according to the age and the body weight, separately took the experimental group and the control group, each group of 10 ewes. Superovulating them and gathering corresponding time blood serum at the same time, the writer used the radioactive immunity analytic method (RIA) to analyze in the serum contents of FSH, LH, E2, P4 and the reductase method to examine the blood serum contents of NO. The writer gained the embryo of surgical operation after they had mated. The test result demonstrates: the number of corpus luteum (17.5±8.38), the number of gained embryos (15.9±8.67) and the number of viable embryos (12.1±5.51) of the experimental group of goats which was markedly higher than the experimental group of sheeps (7.9±2.85,6.8±2.25,4.9±1.20,P<0.01). The control group of Small tail han sheeps the serum content of FSH (the mean value: 3.19±0.312 mIU/mL) in vivo is remarkably higher than the control group of Guanzhong-hybridized goats' level (the mean value: 2.27±0.317 mIU/mL) (P<0.05). Guanzhong-hybridized goats' the serum content of FSH in vivo after using FSH to superovulate Guanzhong-hybridized goats (the mean value: 3.34±0.80 mIU/mL) can have been remarkably elevated (P<0.05), but elevated after using FSH to superovulate Small tail han sheeps' the serum content of FSH in vivo is not remarkable (the mean value: 3.83±0.85 mIU/mL) (P>0.05);In the entire superovlating the goats and sheeps the quite corresponding each blood serum contents ofLH and P4 were not remarkably different (P>0.05).At estrus the serum E2 peak levels (44.19±15.14/7g/wL) of the experimental group of goats were markedly higher than the experimental group of sheeps (32.41±8.62 pg/mL) (PO.05) and positively correlated with the number of corpus luteum, the number of gained embryos, the number of viable embryos of the experimental group of goats and sheeps (P<0.05);At proestrus the serum NO peak levels (17.80±8.99 umol/L) of the experimental group of sheeps were markedly higher than the experimental group of goats (10.93±4.76 umol/L) (PO.05) and negatively correlated with the number of corpus luteum,the number of gained embryos, the number of viable embryos of the experimental group of goats and sheeps (P<0.05);Draws the conclusion by the above result: causing the superovulated effect of Guanzhong-hybridized goats to surpasse the Small tail han sheeps1 reason is Guanzhong-hybridized goats stimulated to extraneous source sex FSH was the more sensitive than Small tail han sheeps. Guanzhong-hybridized goats' the serum contents of FSH increase were remarkable;At estrus the serum E2 peak levels of the experimental group of goats were markedly higher than the experimental group of sheeps. At proestrus the speeds and the net amplitudes Which the experimental group of Small tail han sheeps of the serum levels of FSH were elevated were obviously higher than the Guanzhong-hybridized goats'.Above three aspects reason caused the superovulated distinct effect of goats and sheeps.