| Plant disease by aroused pathogenic microorganism is a focus have been considered by many plant breeders and pathologist in their research since the initiation of cultivating crops. Inducing the Systemic Acquired Resistance (SAR) had already been available to control the disease. Plant disease denfence be elicited by the the elicitors, which is an active topic in plant pathology field. They had an unique advantage: elicitors can induce some special responses in plant and then efficiently cause the plant much resistance to disease. On the contrary, to breed and identify a cultivar of crop with stronger disease-resistance at least required several years and the environmental pollution was often caused by the fungicide prevention.Some removable biological factors as a role of elicitors had been studied as an active topic recently. The main components of ISR including low molecular weight polysaccharide, glycoprotein, polypeptide were removable. Low concentration Verticillium dahliae Kleb toxin was used as the biological factor in our experiments. Verticillium dahliae Kleb as biological factor was creative. The experimental results are devided into four parts.1 The methods in the experimental were improved comparing to the old methods as follows: (1) The shaking time for collecting the root cap cell should be controlled in l-2min, (2)Root cap cell in the culture chamber with darkness was taken 2-6h,(3)The natural red(pH=6.5,0.01% for 3-5min) and Evans blue(0.2% for 3min) as dye stuff were used.The results indicated that with analying living cell of root cap treated by Verticillium dahliae Kleb toxin was a quick way in the room to identify the disease resistance of different cotton verieties.2 The influence that Verticillium dahliae Kleb harmed a root and related anti-enzyme in the degree cotton seedling had been explored. Throw away the paper pot and vermiculite etc. when the cotton seedling was long to go to four leaves, use a water to flush carefully, get:(1) Complete root seedling, (2)Root the remaining root of the lower part 3-5 cm use to disinfect of the knife slice in order to slice a seedling, (3) Stays a main root, cutting off the greatly partsof side root for harm a seedling. Three kinds of different roots cotton seedling that fasten condition immerses to go to by having no germ water dilution a different density in the germ liquid of the 20 ml cotton Verticillium dahliae kleb. Through the 0-48h with the result that the wilt degree and measures the variety of related anti-enzyme Slice a seedling victimizes most seriously and secondly in order to harm a seedling, complete root the out break that fasten the cotton seedling is the most light. Result harmed the cotton seedling exterior pathology in root reaction and the dynamic state bio-chemical reaction of the related enzyme inside the body to carry on a research for the cotton, acquired the plant exterior pathology to respond to test with internal biochemical to fit together mutually.3 Used three low concentration Verticillium dahliae Kleb toxin on the cotton, at the same time, author measured the change of POD activities, SOD activities, MDA contents, accordingly, it is accouted that the 1:50 concentration of Verticillium dahliae Kleb may used as an elicitor which enhances the disease resistance of cotton.4 After used the low concentration toxin, it was found that this toxin could alleviate the invasion process of Verticillium dahliae Kleb and could induce related SAR in cooton.Taken together, It was found that diluted filtrate of Verticillium dahliae Kleb toxin ,could enhance the disease-resistance of cotton .To explore how the Verticillium dahliae Kleb play a role elicitor in Cotton would be very important theoretically and practicably.
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