The Purification And Structure Investigation Of Phellinus Igniariu Water Soluble Polysaccharide

The aim of the present research was to detecte the fermentation technology of Phellinusigniariu mycelium, isolate the polysaccharides of fermental product and presume thepolysaccharides' structure. To detect the fermentation technology, the content of saccharide, protein,oligosaccharide and trace element, the composition of monosaccharide, amino acid and traceelement and the molecular weight distribution of fermental product in different lots weredetermined. The results demonstrated that the composition and content of different lots weresimilar and the technology was stable. The focus of this research was to study the polysaccharides of Phellinus igniariumycelium. A crude polysaccharide was extracted from the fermetal powder of P. igniarius byboiling water. Its content of saccharide and protein was tested by phenol-sulphuric acid andKjeldahl determination. Its composition of monosaccharide was determined by gaschromatography. After purified by deproteinization with enzyme and Sevag method andfractionated by freeze thawing, polysaccharide PIP was obtained. PIP2 was preparated fromPIP using Sepharose CL-6B preparation column. After demonstrated PIP2's homogenicitywith high performance liquid chromatography, its structure was analysis by partial acidhydrolysis, Periodate oxidation, Smith degradation, IR and Methylation.The data obtained indicated that PIP2 was a neutral homoglycan which molecular weightwas 18,000D and glucosidic bonds were β configuration. It had few branch. On average, therewere 1.5 branches every 10 hexose residues. PIP2 possessed a backbone of (1→4)-linkedglucose residues, with substitution at O-3 by (1→3)-linked glucose. The side chains wascomposed of (1→3)-linked glucose with different degree of polymerization. The end wascomposed of Glc.