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Effects Of High Voltage Prick Electric Fields On Sprouts And Activities Of Seeds Of Glycine Max

Posted on:2007-08-01Degree:MasterType:Thesis
Country:ChinaCandidate:H LiuFull Text:PDF
GTID:2133360182998470Subject:Biophysics
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People have paid more and more attention to the biology effects of electric field.Learners at home and abroad have done many researches on biology effects of electrostaticfields especially. And they have reaped the fruit of their researches. For resent years, when weemployed the electrostatic technique to some researches of breeding we have found it canincrease both production and income, can improve the qualities of agricultural products.Glycine max is one of the crops that are planted in large scale in our country. Glycine maxesare the most important raw material of industrials and agricultural production. So there is somuch practical significance to increase the production and improve the quality of the Glycinemax. But now the configuration of electric field is relatively monogenic ,but most of them areplat plates. So there are significances of theory and application to explore the affect ofdifferent electric fields to the growth of crops. This study dealt Glycine maxes in water withhigh voltage prick positive, negative electrostatic field and alternate high voltage prickelectric field. Observed and measured the germination percentage, germination potential,germination index, vitality index, length of sprouts, weight of the fresh and dry sprouts, andsome biochemistry quotas of the activities α-amylase, β-amylase and catalase and the contentof soluble protein.We obtained experiment results as follows:1. Positive high voltage prick electrostaticfield could promote the germination of the Glycine maxes. When the voltage was 2 kV, theelectric field can make the germination percentage, germination index and vitality indeximprove 27.5 %, 151.3 % and 325.2 % separately contract contrast series that were not dealtwith electric field. The activities of α-amylase and the content of soluble protein of the seriesdealt with positive high voltage prick electrostatic field (PESF) exceeded that of thecomparative series remarkably. The series dealt with the voltage of 6 kV had the mostincrease which was 192.4 % for the activity of the α-amylase and 13.4% for the content ofsoluble protein. The electric field firstly promoted and then inhibited the activity of β-amylasewith the increasing of the voltage. 2. High voltage prick negative electrostatic field couldinhibit the germination of the Glycine max in some degree. When the voltage was 6 kV, theNESF had the strongest inhibition which could decrease both the germination percentage andgermination potential 10.0%. There was inhibition of the negative high voltage prickelectrostatic field (NESF) to the activity of α-amylase of the soybeans. The activities ofα-amylase of the series dealt with the voltages of 6 kV and 8 kV were lower than that of thecomparative series remarkably, which were 45.0 % and 62.8 % separately. 3. High voltageprick alternate electric field could promote the germination of the Glycine maxes. Thegermination percentage, germination index and vitality index of the series dealt with thevoltage of 2 kV separately exceeded the comparative series 15.0 %, 51.2 % and 127.4 %especially. There was promotion of the alternate high voltage prick electric field (ACEF) tothe activities α-amylase, β-amylase and catalase and the content of soluble protein in somedegree. The .activity of the catalase was higher 83.9 % and the content of soluble protein washigher 16.1 % of the series dealt with the ACEF than the comparative series most. So weconclude different high voltage prick electric field had different effects on the germination ofGlycine maxes. There were promotions of the PESF to the germination of soybeans. ThePESF promoted the germination percentage, germination index and vitality index remarkablyat macroscopic respect and improved the activities of the α-amylase, β-amylase and catalaseand the content of soluble protein at microcosmic respect. The degree of the promotionchanged according to the voltage of the electric field. There was inhibition of the NESF to thegermination of Glycine maxes. The NESF could make the germination percentage,germination potential etc. decrease in some degree. But it could quicken the growth of thesprouts and promote the activity of some enzymes. There was promotion of the ACEF to thegermination of soybeans. Under some voltage, the ACEF could promote some macroscopicquotas of the germination percentage, germination potential etc. And it also can improve theactivities α-amylase, β-amylase and catalase and the content of soluble. In addition, Differentvoltage of the same electric field had greatly different effect on the germination of Glycinemaxes.
Keywords/Search Tags:Positive high voltage prick electrostatic field (PESF), Negative high voltage prick electrostatic field (NESF), alternate high voltage prick electric field (ACEF), seeds of Glycine max, Germination percentage, Germination potential, Germination index
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