The Effect Of High-voltage Prick Electrostatic Field On Saline-alkali Stress Resistance Of Leymus Chinensis Seedling

Leymus chinensis is the dominant species and and quality forage in Songnen grassland, has good ecophysiological value and great worth of economy. Recently, many plains became saline-alkalized and plain entironment has been destroyed because of the incorrect using of Leymus chinensis plains. So researching deeply the tolerance of Leymus chinensis to salt-alkali stress has significant meaning in both the product of Leymus chinensis and the management and reformation of saline-alkali plains.Leymus chinensis seeds were treated by a variety of electric intensities of high-voltage prick electrostatic field and Leymus chinensis seedling were stressed by various concentration soil water extract in this paper. Proline content, soluble sugar content, inorganic ions contents including Na⁺, K⁺, Ca²⁺, Cl^-, NO₃^-, SO₄^2- and oxalic acid content were determined .The experiment results showed: (1) the proline and solute sugar contents in the leaves and roots of Leymus chinensis seedlings under electric field were more than those under non-electric field. (2) with rising stress intensity, the Na⁺ content in leaves, stems and roots of Leymus chinensis seedlings increased, but the K⁺ content in leaves and stems changed slightly and that in roots decreased obviously. At the same stress condition, the K⁺ content in leaves under15 kV electric field was more 22.4%-38% than that under non-electric field. Besides, SA was little affected by electric field and ST was on the contrary. The ST value under15 kV electric field was higher obviously than that under other electric field conditions in the greater stress condition, and higher 38-60% than that under non-electric field in the same stress intensity. All the Ca²⁺ content decreased with the increasing salinity. The Ca²⁺ content under15 kV electric field was higher more than that non-electric field. In the same stress condition, the Na⁺/K⁺ values in leaves under three electric fields, especially under15 kV electric field, were lower than that under non-electric field, and the Na +/Ca²⁺ values in leaves under 10 kV and 15 kV electric field were lower obviously than that under non-electric field. (3) In the same stress condition, only when electric voltage was 15 kV, NO₃^- content was higher, even reached 1-5 times of that under non-electric field. The ratios of NO₃^-/total anions under three electric field,especially 15 kV electric field, all were higher than those under non-electric field. At the same time, electric field promoted the SO₄^2- accumulation. The results indicated: high-voltage prick electrostatic field promoted Leymus chinensis seedling to accumulate organic osmotic adjustment solutes proline and soluble sugar in cytoplasm of stems and leaves, strengthened the cytoplasm capability of absorbing water and preventing from ion toxicity; affected the osmotic adjustment, ion equilibrium and the metabolism of sodium, kalium, calcium and nitrogen; promoted seedling to absorb K⁺ selectively and inhibit absorption of Na⁺, advanced K⁺ transportation to leaves and Ca²⁺ accumulation specially, reduced Na⁺/K⁺ and Na⁺/Ca²⁺; when electrostatic field voltage was 15 kV, NO₃^- accumulated specially in roots, stems and leaves under salt-alkali stress, the electric field increased the proportion of NO₃^- to the total anions, promoted cell compensation current and ion equilibrium.In summary, Leymus chinensis seeds treated by high-voltage prick electrostatic field have improved seedling adaptation and adjustment capability to salt-alkali stress indeed.