Changes In Protein And Nucleic Acid In The Course Of Dormancy-Broken Of Lilium Brownie Var.viridulum By Low Temperature

In this study, bulbs of Lilium brownie var. viridulum was used as materials and the dynamic content changes of total proteins, hydrophile proteins, DNA, RNA during 5℃ dormancy-broken course were studied. Changes of protein composition were analyzed by SDS-PAGE-Electrophoresis and 2-D- polyacrilamide gel electrophoresis. The acquired protein expression maps were analyzed by software PDQuest with manual aid. Some proteins whose abundance significantly change or specially express during during the cold course were identified by MALDI-TOF analyses. The main results were as folio wings:Change tendency of hydrophilic proteins and hydrophobic proteins is the quite opposite. During the cold storage, the content of soluble proteins has increased to 21.4%. After 30 days' gradual increase it enhanced rapidly and reached a peak value at 60 day and then keep this high level. While for the hydrophobic proteins, they decreased during the early phase and then recovered to the former level.Proteins of low molecular weight are very abundant in the bulbs of Lily and in this experiment it is found that 21kD and 23kD protein bands are the most prominent bands in the electropherogram indicating that they are the most abundant proteins in the bulbs of Lily. After 20 days of cold treatment, the expression of a 95kD protein decreased. After 40 days of cold treatment, the expression of a 17kD protein enhanced significantly.In all, three types of changing proteins were detected during the cold course: I, 5 newly synthesized peptides(34kD/pI4.0, 17kD/pI5.6, 16kD/pI5.4, 16kD/pI4.0 and 95kD/pI7.2);II,4 significantly decreased peptides(4kD/pI6.5, 21kD/pI4.0, 16kD/pI4.8 and22kD/pI5.6);III,11 enormously increased peptides (15kD/pI4.6, 15kD/pI5.0, 16kD/pI4.0, 17kD/pI4.8, 17kD/pI5.8, 35kD/pI3.8, 35kD/pI4.6, 38kD/pI6.4, 48kD/pI6.7, 66kD/pI6.4 and 90kD/pI7.2).A stress and pathogenesis-related protein(Q9ZRU8_FAGSY like) with 17kD/ pI5.6 was specially express after 20 days' low temperature storage. A storage protein (UESY27 like), with 24kD/pI6.5,significantly decreased during cold storage...