Proteins Related With SO₂ Stress In Maize (Zea Mays): An Proteomic Approach

It is of enormous importance for screening and breeding of the plant monitoring SO₂ and the plant resistant to SO₂ to research into the effects of SO₂ on plants at protean level as a whole.In this study, a proteomic approach has been set up to find out proteins related with SO₂ stress in maize. Whole protein of maize leaf (Ludan 981 with and without SO₂ fumigation) were separated by immobilized pH gradient two dimensional polyacrylamide gel electrophoresis and visualized by commassie staining. With the aid of PDQuest 2-DE software, 11 significant protein dots were found out and then 9 of them are pick out to go through peptide mass fingerprinting based on MALDI TOF MS and NCBI database searching . In all, 5 proteins were identified: Ribulose bisphosphate carboxylase [Zea mays], Nucleic acid-binding protein [Zea mays], Ribosomal protection tetracycline resistance protein [Arcanobacterium pyogenes], hypothetical protein FG04726.1 [Gibberella zeae PH-1], nitric oxide synthase [Bombyx mori].This study may provide an insight in to the further research of mechanism of SO₂ resistance in maize.