Analysis Of Storage Proteins And Primary Agronomic Characters In Triticum Compactum Host.

Triticum compactum accessions from different countries were investigated on the primary agronomic characters, and protein contents, and genetic variation of gliadins and high-molecular-weight gluetnin subunits (HMW-GS) were detected by APAGE and SDS-PAGE methods. The main results were described as following:1. The higher variations of gliadin in 70 T.compactum accessions were detected by APAGE. A total of 54 gliadin patterns were observed, among which only 2 bands were shared by all accessions. Eight to 24 bands could be identified in each accession. There were 36,27, 37 and 63 bands at α,β,γ and ω zones, resulting in 12, 10, 9 and 23 gliadin patterns, respectively. The gliadin patterns of 2 accessions were identical, and they could not be distinguished by APAGE.2. Base on the gliadin bands, the genetic similarity (GS) among T. compactum accessions was calculated using NTSYS2.1 software according to the Nei &Li(1979) 's method. Higher genetic diversity among these accessions were detected. The genetic similarity varied from 0.324 to 1.000, with the average of 0.655. The clustering results indicated that genetic relationships in T. compctum based on the gliadin were associated with its geography distributions.3. The composition of high molecular weight subunits (HMW-GS) were identified and analyzed by SDS-PAGE in 63 T.compactum accessions. Twenty-four subunit patterns, resulting from 15 alleles, were found, and the most common subunit patterns were 2*, 7+8, 2+12 and N, 7+8, 2+12 (17.46%). Of the three loci, Glu-Bl had the highest genetic variatious with 10 subunits, among which 7+8, 20 and 21 subunits were observed in higher frequency, which were 39.68%, 22.22% and...