Establishment Of Embryogenic Transformation System In Wheat And Production Of Transgenic Wheat With Salt-responsive Gene.

Mature embryos of wheat varieties R57 (ChuanNongl7) and R59 (ChuanNongl2) which are important cultivar in Sichuan Province were used as explants for culture in vitro to study the effects of conservation on ratio of callus induced from mature wheat embryos .In this work we also researched the effect of individual rye chromosome 1RS on the induction of callus and the regenerating ability of callus cultures in cultured mature embryos of wheat varieties R57 R59 R25(Chuagnnong18) and wheat-rye 1RS/1BL translocation lines constructed with common wheat cultivar MY11(Mianyang 11) and inbred rye lines R12, R3, L155 and "baili" rye. Furthermore, a salt-responsive gene S-adenosylmethionine decarboxylase (SAMDC) gene was transformed to mature embryos of wheat via microprojectile -bombardment. Salt tolerance analysis was implemented to T₁ progeny. In this research, we studied the different factors influencing the biolistic bombardment in wheat transfer and established preliminary wheat mature embryo particle biolistic transformation system.Mature embryos of new wheat variety R57 and R59 , which has been conserved for two years, were used as explant for culture in vitro. The result shows that the ratio of callus induced of the 2-year-conserved is greatly different from the fresh mature embryos. For mature embryos harvested at different time in the same year and had the same conservation time, the mature embryos harvested later had stronger ability of callus formation than the early one.Mature embryos of wheat varieties R57, R59 , R25 and three wheat-rye translocation lines which both have rye IRS chromosome were used as explants for culture in vitro to study the effect of rye chromosomes on callus induction and regeneration in callus cultures of mature embryos. The result showed that, as compared with the control wheat cultivar...