Establishment Of Plant Regeneration System Cultured In Vitro Of Acer Negundo L.

In this study, tissue culture technique and regeneration system for Acer negundo L were established. All theses studies established a base for transforming Acer negundo gene, had important value for cloning aceraceous and other plants.The main contents and results are listed as follows:(1) The disfinfections of explants are different obviously. The best disfinfection of seeding is 1% NaClO₃, 15 min, but adult plant needs 30 min. Adult plant disfinfection is less than seedling's and bacterium ratio is high. Disfinfection with 0.1% HgCl₂ mercury is also good, but the germination ration is bad, albinism ratio is high.(2) The adventitious shoot can be induced from a nodal segments and tip shoots directly. A regeneration protocol through axillary buds has been developed for Acer negundo. Stalk nodus and tip shoot explants were placed on MS medium supplemented with different hormone or different concentration and then compared their ratio of proliferation and differentiation. The result showed that MS medium containing 0.05 mg/L TDZ and 0.05mg/L NAA suited proliferation culture for stalk nodus and tip shoot. The best differentiation medium for them was MS medium with 0.01 mg/L TDZ.(3) A tissue culture system for Acer negundo by the route of inducing calli were primarily established through screening out the best media and hormone combination for different explants(stem segment, nodal segment, petiole and leaf)(4) The plant from tissue culture takes root on MS basic medium easily, but the root is short and there isn't. The best medium for rooting is MS medium with 0.1 mg/L NAA, and the rooting ratio is 88.4%.(5)When tissue culture plant from adventitious shoot, is 3-5cm, the best health young plant were chosen to toughen and transplant in greenhouse. The survival rate is 94.54%.