| The giant panda (Ailuropoda melanoleuca) is one of the most famous endangered animals in the world and has been a symbol for world biodiversity conservation. Compared to other mammals, the new-born pandas have been congenitally immature, which results in high infant mortality . Together with the features of naturally low reproductive rate and small population, the low survival rate resulting from high infant mortality of giant panda makes it more vulnerable to all sorts of threats such as habitat encroachment and destruction. Therefore, it is really important for the conservation of giant panda to develop artificial breeding technology to increase the reproductive rate and lessen the infant mortality. Determination and functional studies of giant panda (Ailuropoda melanoleuca) GH (Am-GH) will contribute to understanding the molecular basis of growth regulation by Am-GH. Furthermore, the production of recombinant Am-GH in eukaryotic expression system would provide a basis for the application of the recombinant GH protein in animal biotechnology.Medicine produced by transgenic plants would have the potential to change the traditional means of production, and to reduce the cost of medicine production. In this paper, pCAMBIA13011-GH, the plant expressing vector consisting of Giant Panda (Ailuropoda melanoleuca) growth hormone gene was constructed, which contains a CaMV 35S promoter and a hygromycin resistance gene, and then transformed Am-GH into tobacco leaf-disc by Agrobacterium-mediated method. A total of 12 regenerated plants with hygromycin resistance were obtained in the selection media. The 12 putative transgenic individuals were tested and 5 of them were verified the presence and integration of the Am-GH into the nuclear genome of tobacco plants by GUS, PCR analysis and Southern blotting. By now, some transgenic plants have already been abloom. Further functional analysis for Am-GH is ongoing. |
PDF Full Text Request