Immune Effect Of Fusion Protein K88ac-STⅡ And VP4-STI To Mice

Recombinant strains were beared and induced by IPTG, detected by SDS-PAGE and Western-blot. The prospective protein was purified with dialytic membrane and the concentration was adjusted to 1mg/ml, then heat-labile enterotoxin B subunit was added into the purified fusion protein to become experimental vaccine, aluminium salt added into as aluminous vaccine.Mice of each groups were vaccinated respectively with experimental vaccine,aluminous vaccine,purified fusion protein (K88ac-STâ…¡/ VP4-STI)and PBS. The serum IgG responses to fusion protein of each groups were detected by indirect ELISA,the Immune Effect of Fusion Protein K88ac-STII and VP4-STI were indicated by the level of antibody and pathological change.As a result, each of recombinant strains can express differential protein, which have the same molecular weight as prospective protein. The differential protein expressed by BL21(DE3)(pET28a-K88ac-STâ…¡)can be identified by anti-K88ac,expressed by BL21(DE3)(pTHioHisB-VP4-STI) can be identified by anti-VP4. the mice immunized with experimental vaccine can produce anti- K88ac-STâ…¡/VP4-STI effectively.while the ileum has no obvious pathological changes.The result indicate that Fusion Protein(K88ac-STâ…¡/VP4-STI) with adjuvant LTB have effective Immune Effect to mice.