The Study For Molecular Marker-assisted Selection Method In Wheat Of Good Subunit And Powdery Mildew Risistance Gene

The gene pyramiding in the material that contained Pm21 and the material that contained subunit pair 5+10 were determined, and the SCAR-based markers linking with powdery mildew resistance gene and the special PCR primers controlling the gene sequence of 1Dy10 and 1Dx5 were used for molecular assisted selection in hybrid offspring. The main results were summarized as followed:1. The result of 1Dx5 and IDylO specific PCR markers was accorded with the result of SDS-PAGE in some materials, also the repetition and speciality of PCR results were good. It indicated that using the 1Dx5 and 1Dy10 special PCR primer to select subunit pair 5+10 was not only feasible, and accurately reliable, and it was effective to select the good quality in wheat and provided the reliable basis on MAS of wheat.2. The MAS efficiency of SCAR₁₄₀₀ in BC₁F₁ between Guinong21 and Neepawa reached 95.3%. The MAS efficiency of SCARhoo in F₃ between JYJY970012 and Ai2002 reached 94.1%. The two efficiency differed 1.2%.The result showed that the SCAR₁₄₀₀ primer linked to Pm21 more closely, then this marker could be conveniently used for marker-assisted selection in wheat breeding programs for the identification or pyramiding of Pm21, and shorten the breeding process.3. In the population of 100 plants F₃ in Guinong21×Neepawa and 50 plants BC₁F₁ in JYJY970012×Ai2002 could select the different subunit combination of plants with resisted powdery mildew gene by the special PCR primers. Thus by way of MAS, it could appraise the purpose gene in early filial generation, and provide the further material of seed selection for the wheat breeding of good subunit and resistanted gene, thus raising the selection efficiency.4. Having the aid of the PCR marker that linked to the resistanted and quality gene to establish MAS System of good subunit and resistanted wheat breeding, it could take the place of MAS to assist selecting the subunit compositions and resistanted gene in early filial generation, and it could greatly increase accuracy and breeding efficiency and speed up the breeding speed of wheat varieties.