Assessment Of Genetic Diversity Of Chewing Cane Germplasm Based On RAPD And ISSR

RAPD and ISSR markers were used to assess the germplasm genetic diversity among 40 individuals of chewing cane, including 39 chewing canes comed from different distracts and Badila, 23 RAPD primers and 28 ISSR primers, with polymorphic and informative patterns, were selected from a total of 100 RAPD ones and 100 ISSR ones to determine these individuals' genetic diversity. The 23 RAPD primers and 28 ISSR primers generated 250 RAPD fragments and 301 ISSR fragments, respectively. For RAPD marker, 175 polymorphic bands were produced, percentage of polymorphic bands was 70%; For ISSR marker, 232 polymorphic bands were produced, percentage of polymorphic bands was 77.1%. Jaccard's genetic similarity matrices and dendrograms for the 40 individuals were formed based on two molecular markers data. In dendrograms, these chewing canes could be divided into four groups: the first group which was proved to be hybrid species contained five individuals such as Baishan, Waigandan, Dudu, wenling chewing canes and artificial hybrid species 474; the second group was Badiia and Fengcengzipichewing cane; the third group only contained one species------Huangpichewing cane; the fourth group was composed of 32 individuals which comed from different provinces such as Fuji, Jianxi, Guizou, Yunnan, Guangxi and so on. The correlation coefficient was 0.9471 between RAPD and ISSR markers. The results revealed that RAPD and ISSR markers were suitable for assessment of germplasm genetic diversity of chewing cane, and ISSR marker was superior to RAPD marker.