Experimental Study On The Effect Of Purple Quartz On Local TGF - α And EGF In Ovariectomized Rats

Objective:This paper explores the material basis of the fluoritum’s influence of the rat models with ovulation failure on transforming growth factor-a (TGF-a), epidermal growth factor (EGF) and ovarian morphology.It provides the theoretical foundation for clinical treatment of ovulation failure.Methods:In this experiment, sixty 24-day-old SD female young rats were chose,each weights 75±5g, and they were divided into six groups at random:model control group, western medicine control group, fluoritum group, calcium fluoride group,norishing kidney prescription, and normal control group, each group had twelve rats. Using the Bogovich method set a pathological rat model of ovulation failure,using the Clomiphene and kidney tonify and blood circulation simulating soup for contracting, and using the immunohistochemicalstaining observed the fluoritum’s influence on the rat with ovarian failure part of theTGF-a and EGF.Result:Comparing with the normal group, the model group’s intensity of expression of TGF-a and EGF is higher. The difference is obvious (p<0.01).After the treatment, the intensity of expression of TGF-a and EGF reduces obviously(p<0.01,p<0.05). It shows that the fluoritum,calcium fluoride,norishing kidney prescription and medicine can repress the intensity of expression of TGF-a and EGF. the above four groups have no difference (p>0.05).This illustrate calcium fluoride and other microelement jointly take effect in reduce the intensity of expression of TGF-a and EGF. The average of fluoritum rats ray density are obvious smaller than calcium fluoride rats.Conclusion:The above result of the experiment indicate that TGF-a and EGF probably participate the nosogenesis of polycystic ovary syndrome (PCOS).Fluoritum can reduce the abnormal high expression in local ovary of ovulation obstruction rat, and attenuate the function of the TGF-a and EGF in local ovary,and it is superior to CaF2 in the intensity. CaF2 and the others elements of fluoritum maybe restrain the level of TGF-a and EGF. This show fluoritum probably have the function of endocrine hormone.It can improve the function of regulate of HPOA, and promote the growth of primary ovarian follicles to dominant follicle...