Expression Of Tumor Invasion - Related Protein In Triple - Negative Breast Cancer And Its Clinical Significance

Background: Breast cancer is the most frequently encountered malignancy and is theleading cause of cancer-related mortality in women worldwide. TNBC is definedimmunohistochemically as breast cancer that does not overexpress human epidermalgrowth factor receptor2((HER2, ERBB2) and is estrogen receptor (ER) andprogesterone receptor (PR) negative. As a special subtype, TNBC becomes atreatment difficulity because of its unique molecular and clinical features. Severerbiological aggressive behavior, together with earlier recurrence and metastasis is thekey reason why TNBC ofen fails to treat. Distant metastasis is the leading causes ofdeath of breast cancer. Tumor metastasis is a complex interaction between tissues andtumor cells and is a continuous process in which a number of highly orderedmolecules participate in. A variety of tumor invasion related proteins are involved inthe process of invasion and metastasis of tumor. Studies have shown that the jointinspection of tumor molecular markers plays an important role in the early diagnosisand treatment, disease progression and prognosis.Tumor invasion related proteins(N-cadherin CD44v6MUC1MMP2and VEGF) are thought to be associated withthe occurrence, proliferation, metastasis, and prognosis of tumor, but the clinicalsignificance of their expression in TNBC are not complete.Objective: This experiment investigates the expression of tumor invasion relatedproteins (N-cadherin CD44v6MUC1MMP2and VEGF) in triple negative breastcancer (TNBC) and non-triple breast cancer.By analyzing the correlation betweentheir expression with related clinicopathological parameters, this experiment mayprovide theory basis for invasion and metastasis evaluation, and exploring targetedtherapy in triple negative breast cancer.Method:104cases of triple negative breast cancer and168cases of non-triplenegative breast cancer were collected by purchasing breast cancer tissue microarray.All specimens were procided with specific pathological diagnosis and ER, PR, HER-2immunohistochemical detection. An immunohistochemical method (steptavidinperoxldase,SP) was used to detect the expressions of tumor invasion related proteins((N-cadherin CD44v6MUC1MMP2and VEGF) in triple negative breast cancer(TNBC) and non-triple breast cancer (non-TNBC), and some clinical and pathologicalparameters were collected. SPSS16.0statistic software was applied to analyze thesedata. Results:(1) The expression of N-cadherin, CD44v6, MUC1and MMP2shows nostatistically significant differences between TNBC and non-TNBC. The expression ofVEGF was significantly higher in non-TNBC than in TNBC (P=0.023).(2) Nocorrelations were found between the expression of the tumor invasion related proteins(N-cadherin, CD44v6, MUC1, MMP2and VEGF) with age, histologic grade, tumorsize, lymphatic metastasis, clinical stage in TNBC; In non-TNBC, except thesignificant correlation between CD44v6expression and absence of lymph nodemetastasis (P=0.023), no correlations were found between the expression of thosesproteins and the other related clinicopathological characteristics.(3) In the104casesof TNBC, N-cadherin and MMP2, CD44v6and MUC1,CD44v6and MMP2, MUC1and VEGF MUC1and MMP2show a positive correlation with each other, which mayindicted that these tumor invasion related proteins have close relations in the progressof the tumor.Conclusion: In our study, the tumor invasion related proteins (N-cadherin, CD44v6,MUC1, MMP2and VEGF) igh expression in TNBC than in non-TNBC,in terms of no correlations between their expression and prognosis related factors suchas histologic grade, lymphatic metastasis, clinical stage, their roles served asprognosis indicators in TNBC need further validation.