Effects Of Massage On Expression Of MBP And LN In Ridge And Nerve Of SNI Rats

[Objective]Elucidate part of the mechanism of tuina in the treatment of peripheral nerve injury by studying the effect of tuina treatment on laminin (LN) and myelin basic protein (MBP) expression and behavioral change in sciatic nerve injured rats, as well as improve the basic theory of tuina in the treatment of peripheral nerve injury and assist in the progress and development of the academic field of tuina.[Methods]Using peripheral nerve injured rats as a model, daily treatments were performed with a "tuina simulator" applying point pressing method→pluckingmethod→circular rubbing methods in succession stimulating the acupuncture points BL56, BL 57, GB 34 in succession on the operated side. Behavior changes, nerve and muscle feeling after tuina therapy and movement recovery were observed. Two key parts of the spinal cord related to the damaged area were taken for morphological observation. The expression of LN at the point of injury and in the spinal cord was measured, as well as the expression of MBP at the point of injury. These proteins and their relationship with the myelin repair were analyzed. And finally, a comprehensive analysis of the mechanism of tuina in the effective treatment of peripheral nerve injury and its molecular pathway was performed.[Results]1. The behavioral science resultsThe behavioral science gastrocnemius muscle wet weight experiment showed:seven days after modeling, the wet weight of the injured gastrocnemius muscles of the model group rats were lower than the normal group and control group (P<0.05), indicating that after sciatic nerve injury there is a certain amount of muscle damage, the injured gastrocnemius exhibited impaired nutrition, loss of innervation, and muscle atrophy. Using tuina therapy after 20 days, the injured gastrocnemius muscle wet weight and muscle atrophy showed a certain amount of improvement in the nerve injured rats, though still showing a difference from those of the normal group (P< 0.05). These results indicate the tuina can promote the repair of damaged nerves, and can also improve post-injury atrophy.2. The morphological resultsHE nerve dyeing observations:seven days after modeling, the normal group and the sham-operated group showed complete cellular structure and well-arranged Schwann cells. The model group and model control group showed disorder nerve fibers,schwann cells death and axon disintegration. The Schwann cells of the Tuina group returned to basically normal, the axons were clear, nerve fiber arrangement was basically normal.20 days after modeling, the model group and the model control group showed nerve fiber swelling, scattering, nerve demyelination and axonal collapse, and the myelin sheath and axon mixed to form oval-shaped, Schwann cell proliferation occured.3. The results of MBP in the spinal cord and nervesSeven days after modeling, the model group showed thehighest MBP expression, and showed a significant difference when compared with the normal group and control group (P<0.05). Twenty days after treatment, the model group and control group showed thehighest MBP expression, and showed a significant difference when compared with the normal group (P< 0.05). The MBP expression in the tuina group was restored to normal levels, showing significant difference when compared with model group (P < 0.05), and showing no significant difference when compared with normal group (P> 0.05).4) LN expression in the spinal cord and nervesSeven days after modeling, the LN expression was the highest in the model group, and showed significant difference when compared with the normal group and control group (P<0.05). After 20 treatments, the expression of LN in the model and control groups increased and was significantly different when compared with normal group (P<0.05), but the expression in the tuina group rose the highest, showing significant differences when compared with both the control group and the normal group (P<0.05);[Conclusion]1 Tuina can improve muscle strength in the gastrocnemius muscle of sciatic nerve injured rats, prevent deformation and contracture of limb musculature, and can also improve the rats’pain sensitivity thus promoting recovery of nerve and muscle feeling and motor function.2 Tuina can protect the neuron cell body, and improve Nissle body disappearance, reduction of synaptic terminals and nucleus migration, thus promoting restoration of the neural axon and ability regeneration.3 Tuina can increase the expression of LN in the damaged area, and promote the proliferation of Schwann cells, it can also can improve the expression of MBP in the spinal cord and the damaged area, as well as reducing the loss of MBP. From the perspective of the myelin regeneration, tuina therapy can thus achieve peripheral nerve injuryrepair via this mechanism.