Study On The Relationship Between Microenvironment Immune - Related Factors And Prognosis Of Patients With

Hepatocellular Carcinoma is one of the most common malignanat cancer,According to the official data, the incidence of HCC ranks fifth which is about5.7% of total cancer. According to the latest news, every year the number increase748.000 all over the world.China is the highest incidence of HCC country, the number of HCC patients is more than a half of total HCC patient in the world which is just behind Lung cancer as the second.With the developing of the society and technology, the research and treatment on HCC have achieved great progress, but the death of HCC is still high. No matter in east or west, the5-year recurrence rate is as high as 60%-70%. Therefore,making the study on recurrence and metastasis of HCC is very meaningful and important.The recurrence and metastasis of HCC is a multi-stage, multigenes-related,motional process. Including invasion into blood, survive in circulation.Tumor microenvironment plays an important role in the recurrence and metastasis of HCC which consist of tumor cells,endothelial cells, immune cells,structure cells of tumor vessels and so on.Some research find the immunity genes of Tumor microenvironment is the most accurate independent factor for the prognosis of HCC patients which is better than TNM stages.Frist we study the influence of immune cells for prognosis of HCC patients on the gene level. We select two group patients in different period randomly. The number of group A is 376 and group B is 192. All of them was the first time had surgery in Huashan Hospital. In group A, we use PCR to analysis the gene expression and construct a immunity genes related model. In group B, we use microarray and immunohistochemical technique to analysis the related protein.And validation the Stability, effectiveness and versatility of the model in group B.