| Objective: To find valid prognostic marker for hepatocellular carcinoma (HHC) patients through studying the correlation between CD 105 or CD34 expression and postoperative recurrence and metastasis as well as to explore some special molecular pathological features and biological behavior of solitary large HCC.Methods: 113 HCC cases with paracarcinomatous tissue and 14 normal liver tissue specimen were stained with CD 105, CD34, VEGF by immunohistochemistry method. Quantitation of micro-vessels which was highlighted by anti-CD 105 mAb and anti-CD34 mAb and semi-quantitation of VEGF expression was performed and analyzed in conjunction with the clinical-pathological characteristics and the result of follow-up.Results: Negative expression of CD 105 on vascular endothelial in normal liver tissue (100%, 14/14) and paracarcinomatous liver tissue (100%, 113/113) was found, while the expression on vascular endothelial in HCC tissue were positive (100%, 113/113). CD34 expression, on the other hand, was found positively in normal liver tissue (86.7%, 12/14) and paracarcinomatous liver tissue (93.8%, 106/113) as well as in HCC tissue (100%, 113/113). The micro-vessel density evaluated by an anti-CD 105 monoclonal antibody (CD105-MVD) and an anti-CD34 monoclonal antibody (CD34-MVD) were 71.7?.3 and 106.3?0.4, respectively. There was statistically significant correlation between CD105-MVD and CD34-MVD (r=0.248,P = 0.021). CD34-MVD was significantly correlated with VEGF expression (r=0.243,P=0.024), while CD105-MVD was more closely correlated with VEGF expression (r= 0.300, P=0.005)o There proved to be no correlation between VEGF expression and micro-vessels that were highlighted by anti-CD34 mAb and were not highlighted by anti-CD 105 mAb. The correlation between CD105-MVD values and microscopic venous invasion were statistically significant (r=0.254, P=0.018), while we didn't find any significant correlation between CD15-MVD and liver cirrhosis, tumor diameter,incapsule formation, Edmondson's grade , preoperative alpha-fetoprotein (AFP) concentration .There was no significant correlation between CD34-MVD and any such clinical-pathological features. The 2-year survival rate of all HCC patients was 30.1%. The 2-year survival rate of lower CD105-MVD (CD105-MVD<56, the median values) HCC patients was 47.1%, significantly higher than that of higher CD105-MVD HCC patients (13.5%, P=0.014). Whereaas the 2-year survival rate of lower CD34-MVD (CD105-MVD<94, 33.3%) HCC patients also seemed to be higher than that of higher CD34-MVD HCC patients (26.9%), the difference did not reach a statistical significance (P=0.601). The 2-year survival rate of HCC patients whose specimen had been found microscopic venous invasion was 14.8%, significantly lower than that of HCC patients whose specimen hadn't (46.9%, P=0.031), while we could not find any other clinical-pathological characteristics involved in this study influent the 2-year survival rate of HCC patients significantly. When compared to nodular HCC, cell differentiation was better, capsule formation was more easily, microscopic venous invasion occurred less frequently, associated liver cirrhosis was milder in solitary large HCC. The CD105-MVD in solitary large HCC and in nodular HCC were 60.42?0.55 and 82.58?.41, respectively, and the difference between them was significant (P=0.021), while the difference between CD105-MVD in solitary large HCC and in small HCC (54.88?.81) was not (P=0.352). The 2-year survival in solitary large HCC patients (55.0%) and small HCC patients (52.2%) were all higher than that of nodular HCC (13.3%), and the difference between solitary large HCC and small HCC didn't reach a statistical significance (P=0.584). CD105-MVD, but not CD34-MVD, could significantly influent the 2-year survival rate of HCC patients in each groups. Microscopic venous invasion could significantly influent the 2-year survival rate of small HCC patients and solitary large HCC patients (P=0.025 and P=0.019, respectively) except nodular HCC patients (P=0.912), while we...
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