| Research Purposes:Renal osteodystrophy is one of the common complications of the end-stage chronic renal disease. High parathyroid hormone leads to secondary hyperparathyroidism and calcium and phosphorus metabolism disorders, which cause a secondary range of reactions of bone marrow superssion, vascular calcification, cardiovascular disease, exacerbation of chronic renal failure progress. The Traditional Chinese Medicine has played a unique role in the treatment of renal osteodystrophy.In this study, adenine suspension combined with high phosphorus food was given orally to establish rat model with renal osteodystrophy and secondary hyperparathyroidism. We investigated and tried to explore the mechanism of the BUSHENHUOXUE Docation(BSHX) treating model rats in general status, PTH and regulation of bone mineral metabolism.Research Methods:1. Methods and Interventions:90 Sprague-Dawley male rats, weighting 200-230g, were randomly divided into model group, TCM group and nomal group. Adenine suspension 250mg/kd-d was given orally to the model group and BSHX group at 9 am.. BSHX herbs were boiled and concentrated to 4g crude herbs in 1 milliliter decotion. TCM group was given BSHX four times than clinical adult dosage according to the weight while model group was given normal saline. TCM group and model group were fed by high-Pi diet(1.2% P). The adenine suspension lasted 6 weeks and half dosage for next 2 weeks. BSHX lasted for 8 weeks.2. Observation indexes:Recorded mental state, changing of weight and quantity of death at 0 day and 8th week. After killing, calculated the kidney orgen index. Biochemical immunization was used to detect the blood creatinine(CRE), blood urea nitrogen(BUN), calcium(Ca), serum phosphorus(P), parathyroid hormone(PTH). ELISA was used to detect parathyroid hormone(PTH), bone-specific alkaline phosphatase(BAP), tartrate resistant acid phosphatase(TRAP).Results:1. Indicators of renal function:The blood creatinine(CRE) and blood urea nitrogen(BUN) of TCM group were lower than model group. There were apparent statistical differences between groups(p=0.000<0.01).2. Bone mineral metabolism:The serum phosphorus(P) of TCM group was lower than the model group. There was a apparent statistical difference between groups(p=0.000<0.01). The calcium(Ca) of TCM group is higher than model group. There was a apparent statistical difference between groups(p=0.001< 0.01). Compared to the model group, the parathyroid hormone(PTH) of TCM group is lower. There was a statistical difference between groups(p=0.017<0.05).3. Weight and kidney indexes:The rats of TCM group was heavier than model group. There was a apparent statistical difference between groups(p=0.003<0.01). The kidney indexes of TCM group was lower than model group. There was a apparent statistical difference between groups(p=0.000<0.01).4. Expression of PTH, OPG/RANK/RANKL mRNA:The OPG expression of TCM group was lower than the model group with a apparent statistical difference(p=0.001<0.01). RANKL and PTH expressed in TCM group were lower than model group with apparent statistical differences(p=0.000<0.01). The RANK expressed no statistical differences between two groups(p=0.940>0.01).5. Expression of inflammatory cytokines:IL-1, IL-6 and TNF-a expressed in TCM group were lower than model group with apparent statistical differences(p=0.000<0.01).6. Bone mineral metabolism(ELISA):PTH(p=0.025<0.05) and BAP(p=0.033<0.05) of TCM group were lower than model group with statistical differences. There was no differences between two groups in TRAP(p=0.333>0.05).Conclusions:1. BSHX could reduce CRE, BUN,PTH and SHPT of renal osteodystrophy rats induced by adenine.2. BSHX could mitigate kidney injury induced by adenine.3. BSHX could reduce P and PTH of renal osteodystrophy rats and increase Ca to regulate the bone mineral metabolism disorders.4. BSHX could inhibit hyperfunction of osteoclasts in high turnover bone disease, inhibit osteoblasts over-secretion to mitigate high bone turnover.
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