| Object:The recovery of the brain blood flow and angiogenesis are critical to the improvement of brain function after cerebral ischemia. The present study is aimed to investigate whether enriched environment could improve the recovery of the brain blood flow and angiogenesis after cerebral ischemia.Methods:Rats were randomly divided into three groups:Sham group (Sham group), Standard Condition group (SC group) and Enriched Environment group (EE group). Then all the rats were operated by Middle Cerebral Artery Occlusion (MCAO), but there is no occlusion in sham group. The middle cerebral artery was occluded for 1.5 h, followed by reperfusion. Then rats were reared in EE and SC condition respectively for 4 weeks. The cerebral blood flow supplied by MCA of rats was detected by laser speckle imaging (LSI) before,1 h and 4 weeks after MCAO. In order to detect the cerebral vessel density in ischemic area, fluorescein isothiocyanate (FITC)-dextran was administered intravenously to the ischemic rats, then the brains were removed from the severed heads about 5 minutes later and brain sections was obtained and fixed, then the brain sections were analyzed with a laser-scanning confocal imaging system. Additionally, the protein expression of VEGF is detected by Western-blot.Results:Compared with the cerebral blood flow before occlusion, the CBF of EE group and SC group in infarct area 1 h after occlusion was decreased significantly (P<0.01), which has no obvious change in sham group. The CBF of EE group in infarct area 4 weeks after occlusion was increased significantly compared with the SC group (P<0.05). Moreover, the vessel surface area in the ischemic hemisphere of EE group increased significantly compared with those of the SC group (P<0.05). In addition, compared with the SC group, the protein expression of VEGF in EE group was increased significantly (P<0.05).Conclusion:EE could increase the CBF in the ischemic area of the rats after cerebral ischemia, which possibly has relevant relationship with the higher cerebral blood vessel density induced by the greater protein expression of VEGF.Object:Angiogenesis is the major type of vascularization after cerebral ischemia in adult, and angiogenesis has become the main trend in vascularization research. Brain microvascular endothelial cell is the primary type of cerebral vascular endothelial cell and has great importance to angiogenesis after cerebral ischemia. The present study is aimed to investigate whether the serum from rats following enriched environment intervention has proliferation effect on brain microvascular endothelial cell after oxygen glucose deprivation.Method:The intervention of enriched environment and standard environment was done ditto. Ventricular blood collection method was implemented after intervention, then the blood was centrifugal and the supernatant was obtained and filtered. The brain microvascular endothelial cell was cultured and OGD model was implemented to mimic the cerebral ischemia in vitro. Then preliminary experiment was undertaken:culture the BMECs after OGD by 10%,20% and 40% intervened serum respectively and detected cell proliferation by CCK-8 to fumble about the optimal concentration. Finally, culture the BMECs after OGD using the optimal concentration serum so as to investigate the proliferation effect further.Results:The OD value detected by CCK-8 is significantly greater in BMECs cultured by 10% intervened serum than that of 20% and 40% intervened serum (P<0.01). Additionally, the OD value detected by CCK-8 is significantly greater in BMECs cultured by 10% EE serum than that of 20% SC serum (P<0.01).Conclusion:The proliferation effect of 10% intervened serum on BMECs after OGD is the most significant. Furthermore, the proliferation effect of 10% EE serum on BMECs after OGD is significantly better than that of the 10% SC serum. |
